Lipid specificity and location of the sterol carrier protein-2 fatty acid-binding site: A fluorescence displacement and energy transfer study

被引:52
作者
Frolov, A
Miller, K
Billheimer, JT
Cho, TH
Schroeder, F
机构
[1] TEXAS A&M UNIV,DEPT PHYSIOL & PHARMACOL,TVMC,COLLEGE STN,TX 77843
[2] DUPONT CO INC,EXPT STN,CARDIOVASC DEPT,WILMINGTON,DE 19898
关键词
D O I
10.1007/s11745-997-0154-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although it was recently recognized that sterol carrier protein-2 (SCP-2) interacts with fatty acids, little is known regarding the specificity of SCP-2 for long-chain fatty acids or branched-chain fatty-acid-like molecules. Likewise the location of the fatty-acid binding site within SCP-2 is unresolved A fluorescent cis-parinaric acid displacement assay was used to show that SCP-2 optimally interacted with 14-22 carbon chain lipidic molecules: polyunsaturated fatty acids > monounsaturated, saturated > branched-chain isoprenoids > branched-chain phytol-derived fatty acids. In contrast, the other major fatty-acid binding protein in liver, fatty-acid binding protein (L-FABP), displayed a much narrower carbon chain preference in general: polyunsaturated fatty acids > branched-chain phytol-derived fatty acids > 14- and 16-carbon saturated > branched-chain isoprenoids. However, both SCP-2 and L-FABP displayed a very similar unsaturated fatty-acid specificity profile. The presence and location of the SCP-2 lipid binding site were investigated by fluorescence energy transfer. The distance between the SCP-2 Trp(50) and bound cis-parinaric acid was determined to be 40 Angstrom. Thus, the SCP-2 fatty-acid binding site appeared to be located on the opposite side of the SCP-2 Trp(50) These findings not only contribute to our understanding of the SCP-2 ligand binding site but also provide evidence suggesting a potential role for SCP-2 and/or L-FABP in metabolism of branched-chain fatty acids and isoprenoids.
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页码:1201 / 1209
页数:9
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