Isolation and structural characterization of sugarcane bagasse lignin after dilute phosphoric acid plus steam explosion pretreatment and its effect on cellulose hydrolysis

被引：73

作者：

Zeng, Jijiao ^{[1
]}

Tong, Zhaohui ^{[1
]}

Wang, Letian ^{[1
]}

Zhu, J. Y. ^{[2
]}

Ingram, Lonnie ^{[3
]}

机构：

[1] Univ Florida, IFAS, Dept Agr & Biol Engn, Gainesville, FL 32611 USA

[2] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA

[3] Univ Florida, Dept Microbiol & Cell Sci, Gainesville, FL 32606 USA

来源：

BIORESOURCE TECHNOLOGY | 2014年 / 154卷

基金：

美国食品与农业研究所;

关键词：

Lignin structure; Soluble lignin; Nuclear magnetic resonance (NMR); Cellulose hydrolysis; LIGNOCELLULOSIC BIOMASS; BIOFUELS; POPLAR; STRAW; NMR;

D O I：

10.1016/j.biortech.2013.12.072

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The structure of lignin after dilute phosphoric acid plus steam explosion pretreatment process of sugarcane bagasse in a pilot scale and the effect of the lignin extracted by ethanol on subsequent cellulose hydrolysis were investigated. The lignin structural changes caused by pretreatment were identified using advanced nondestructive techniques such as gel permeation chromatography (GPC), quantitative C-13, and 2-D nuclear magnetic resonance (NMR). The structural analysis revealed that ethanol extractable lignin preserved basic lignin structure, but had relatively lower amount of beta-O-4 linkages, syringyl/guaiacyl units ratio (S/G), p-coumarate/ferulate ratio, and other ending structures. The results also indicated that approximately 8% of mass weight was extracted by pure ethanol. The bagasse after ethanol extraction had an approximate 22% higher glucose yield after enzyme hydrolysis compared to pretreated bagasse without extraction. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：274 / 281

页数：8

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