The Solubility of Orthorhombic Lysozyme Crystals Obtained at High pH

被引：46

作者：

Aldabaibeh, Naser ^{[1
,2
]}

Jones, Matthew J. ^{[1
]}

Myerson, Allan S. ^{[2
]}

Ulrich, Joachim ^{[1
]}

机构：

[1] Univ Halle Wittenberg, Zentrum Ingn Wissensch, Verfahrenstech TVT, D-06099 Halle, Saale, Germany

[2] IIT, Dept Biol & Chem Engn, Chicago, IL 60616 USA

来源：

CRYSTAL GROWTH & DESIGN | 2009年 / 9卷 / 07期

基金：

美国国家卫生研究院;

关键词：

EGG-WHITE LYSOZYME; CRYSTALLIZATION; PROTEIN; TEMPERATURE; GROWTH; POLYMORPHISM; MECHANISMS; MOLECULES;

D O I：

10.1021/cg900113e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The high pH region of the phase diagram of lysozyme with NaCl as a precipitant was determined. In this region of the phase diagram, lysozyme crystallizes in one of two different orthorhombic modifications, the low and high temperature orthorhombic modifications. The solubility of two modifications was measured at different temperatures, pH values, and NaCl concentrations. Both modifications show a similar dependence on the solution conditions where solubility increases with temperature and decreases with pH and NaCl concentration. The transition temperature between the two modifications was determined from the solubility curves and was shown to increase with pH and NaCl concentration. At pH values close to the isoelectric point (pH 11), the transition temperature becomes independent of NaCl concentration.

引用

页码：3313 / 3317

页数：5

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