Complete nucleotide sequences and gene organization of mitochondrial genome of Bufo gargarizans

被引：60
作者：
Cao, Sen-Yang
Wu, Xiao-Bing [1 ]
Yan, Peng
Hu, Yu-Ling
Su, Xia
Jiang, Zhi-Gang
机构：
[1] Anhui Normal Univ, Life Sci Coll, Wuhu 241000, Peoples R China
[2] Anhui Key Lab Conservat & Exploitat Biol Resource, Wuhu 241000, Peoples R China
[3] Chinese Acad Sci, Inst Zool, Beijing 100080, Peoples R China
来源：
MITOCHONDRION | 2006年 / 6卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Bufo gargarizans; mitochondrial genome; phylogenetic tree;
D O I：
10.1016/j.mito.2006.07.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The complete mitochondrial genome of Bufo gargarizans was sequenced using overlapping polymerase chain reaction (PCR) amplicons (GenBank Accession No. DQ275350). The genome is 17,277 base pairs in length, containing 13 protein-coding genes (ATP6, ATP8, COI-III, NDI-6, ND4L, Cyt b), 2 ribosomal RNAs (12S rRNA and 16S rRNA), 22 transfer RNAs and a putative control region. We analyzed the sequence using bioinformatics methods comparing the obtained mtDNA sequence with other toads and frogs. Based on the concatenated nucleotide sequences of protein-coding genes, we constructed a phylogenetic tree with maximum likelihood (ML) and maximum parsimony (MP) methods and discussed the phylogenetic relationships among 11 species of Anura. (c) 2006 Elsevier B.V. and Mitochondria Research Society. All rights reserved.
引用
收藏
页码：186 / 193
页数：8
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