MIWI-independent small RNAs (MSY-RNAs) bind to the RNA-binding protein, MSY2, in male germ cells

被引：20

作者：

Xu, Mingang ^{[1
]}

Medvedev, Sergey ^{[2
]}

Yang, Juxiang ^{[1
]}

Hecht, Norman B. ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Ctr Res Reprod & Womens Hlth, Dept Obstet & Gynecol, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 30期

关键词：

noncoding RNA; RNA-protein interactions; Y box proteins; spermatogenesis; NONCODING RNAS; MESSENGER-RNA; TESTIS; PIRNAS; TRANSCRIPTION; EXPRESSION; ABSENCE; CLIP;

D O I：

10.1073/pnas.0903944106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The germ cell-specific DNA/RNA-binding protein MSY2 binds small RNAs (MSY-RNAs) that are approximate to 25-31 nt in length, often initiate with a 5' adenine, and are expressed in both germ cells and somatic cells. MSY-RNA levels do not decrease in Miwi or Msy2 null mice. Most MSY-RNAs map within annotated genes, but some are PIWI-interacting RNA (piRNA)-like and map to piRNA clusters. MSY-RNAs are in both nuclei and cytoplasm. In nuclei, MSY-RNAs are enriched in chromatin, and in the cytoplasm they are detected in both ribonucleoproteins and polysomes.

引用

页码：12371 / 12376

页数：6

共 9 条

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Yang, JX
Medvedev, S
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Schultz, RM
Hecht, NB
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (05) : 1513 - 1518
[2] Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertility
Yang, JX
Medvedev, S
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Tang, LC
Agno, JE
Matzuk, MM
Schultz, RM
Hecht, NB
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (16) : 5755 - 5760
[3] A sequence-specific RNA binding complex expressed in murine germ cells contains MSY2 and MSY4
Davies, HG
Giorgini, F
Fajardo, MA
Braun, RE
DEVELOPMENTAL BIOLOGY, 2000, 221 (01) : 87 - 100
[4] In the absence of the mouse DNA/RNA-binding protein MSY2, messenger RNA instability leads to spermatogenic arrest
Yang, Juxiang
Morales, Carlos R.
Medvedev, Sergey
Schultz, Richard A.
Hecht, Norman B.
BIOLOGY OF REPRODUCTION, 2007, 76 (01) : 48 - 54
[5] Deletion of the DNA/RNA-binding protein MSY2 leads to post-meiotic arrest
Yang, Juxiang
Medvedev, Sergey
Yu, Junying
Schultz, Richard M.
Hecht, Norman B.
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2006, 250 (1-2) : 20 - 24
[6] The RNA-Binding Protein IGF2BP1 Marks Germ Cells but Is Dispensable for Mouse Fertility
Wang, Shu
Wu, Shan
Tang, Jinyan
Chen, Yuan
Zhang, Yiyun
Long, Wenwu
Wu, Xin
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2025, 92 (02)
[7] The DNA/RNA-binding protein, TB-RBP, moves from the nucleus to the cytoplasm and through intercellular bridges in male germ cells
Morales, CR
Wu, XQ
Hecht, NB
DEVELOPMENTAL BIOLOGY, 1998, 201 (01) : 113 - 123
[8] Elevated levels of the polyadenylation factor CstF 64 enhance formation of the 1kB testis brain RNA-binding protein (TB-RBP) mRNA in male germ cells
Chennathukuzhi, VM
Lefrancois, S
Morales, CR
Syed, V
Hecht, NB
MOLECULAR REPRODUCTION AND DEVELOPMENT, 2001, 58 (04) : 460 - 469
[9] CYTOPLASMIC PROTEIN-BINDING TO HIGHLY CONSERVED SEQUENCES IN THE 3' UNTRANSLATED REGION OF MOUSE PROTAMINE-2 MESSENGER-RNA, A TRANSLATIONALLY REGULATED TRANSCRIPT OF MALE GERM-CELLS
KWON, YK
HECHT, NB
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) : 3584 - 3588

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