Rat brain proteome in morphine dependence

被引：41

作者：

Bierczynska-Krzysik, Anna

Bonar, Emilia

Drabik, Anna

Noga, Marek

Suder, Piotr

Dylag, Tomasz

Dubin, Adam

Kotlinska, Jolanta

Silberring, Jerzy

机构：

[1] Jagiellonian Univ, Fac Chem, Dept Neurobiochem, PL-30060 Krakow, Poland

[2] Jagiellonian Univ, Fac Chem, Reg Lab, PL-30060 Krakow, Poland

[3] Jagiellonian Univ, Fac Biotechnol, PL-30060 Krakow, Poland

[4] Jagiellonian Univ, Coll Med, Inst Med Biochem, PL-30060 Krakow, Poland

[5] Tech Univ Lodz, Inst Tech Biochem, Fac Food Sci & Biotechnol, PL-90924 Lodz, Poland

[6] Med Acad, Dept Pharmacokinet, Lublin, Poland

[7] Polish Acad Sci, Ctr Polymer Chem, Zabrze, Poland

来源：

NEUROCHEMISTRY INTERNATIONAL | 2006年 / 49卷 / 04期

关键词：

morphine dependence; proteome; mass spectrometry; rat brain; protein profiling;

D O I：

10.1016/j.neuint.2006.01.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to reveal potential markers associated with drug dependence, using the proteornic approach. Gels containing samples derived from morphine-treated and control animals were compared and analyzed. Inspection of protein profiles, following TCA/acetone precipitation and the use of nano-scale liquid chromatography coupled to tandem mass spectrometry, allowed for identification of eleven potential dependence markers, mainly cytoplasmic and mitochondrial enzymes, e.g. proteins that belong to GTPase and GST superfamilies, ATPase, asparaginase or proteasome subunit p27 families. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：401 / 406

页数：6

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