Antioxidant Enzymes and Heat Shock Protein Genes from Liposcelis bostrychophila Are Involved in Stress Defense upon Heat Shock

被引:36
作者
Miao, Ze Qing [1 ,2 ]
Tu, Yan Qing [1 ,2 ]
Guo, Peng Yu [1 ]
He, Wang [1 ,2 ]
Jing, Tian Xing [1 ,2 ]
Wang, Jin Jun [1 ,2 ]
Wei, Dan Dan [1 ,2 ]
机构
[1] Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400715, Peoples R China
[2] Southwest Univ, Acad Agr Sci, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
psocids; stored product pest; oxidative stress; heat treatment; expression profile; LIPID-PEROXIDATION; THERMAL-STRESS; EXPRESSION; RESPONSES; LEPIDOPTERA; TEMPERATURE; HSP70; COLEOPTERA; PSOCOPTERA; TOLERANCE;
D O I
10.3390/insects11120839
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Simple Summary Liposcelis bostrychophila is one of the most serious pests of stored commodities among the psocids. Controlling psocids mainly relies on chemical insecticides and heat stress. In fact, L. bostrychophila has developed high levels of resistance or tolerance to heat treatment in grain storage systems. In this study, we evaluated the changes in malondialdehyde (MDA) concentration after different high temperatures. The result showed that MDA is increased slightly overall, but a drastic increase is detected at 42.5 degrees C for exposure of different times. To further explore the principles of L. bostrychophila in response to heat stress, we tested the changes of superoxide dismutase (SOD), catalase (CAT), peroxidases (POD) and glutathione-S-transferases (GST) activities under different heat treatments and identified four inducible LbHsp70 genes and one LbHsp110 gene. Enzyme activities and transcript levels changed drastically after different heat treatments. These findings contribute to our understanding of the mechanism of L. bostrychophila responding to heat stress and provide baseline information for further understanding the excellent targets of L. bostrychophila. Psocids are a new risk for global food security and safety because they are significant worldwide pests of stored products. Among these psocids, Liposcelis bostrychophila has developed high levels of resistance or tolerance to heat treatment in grain storage systems, and thus has led to investigation of molecular mechanisms underlying heat tolerance in this pest. In this study, the time-related effects of thermal stress treatments at relatively high temperatures on the activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidases (POD), glutathione-S-transferases (GST) and malondialdehyde (MDA), of L. bostrychophila were determined. Thermal stress resulted that L. bostrychophila had a significantly higher MDA concentration at 42.5 degrees C, which indicated that the heat stress increased lipid peroxidation (LPO) contents and oxidative stress in this psocid pest. Heat stress also resulted in significant elevation of SOD, CAT and GST activities but decreased POD activity. Our data indicates that different antioxidant enzymes contribute to defense mechanisms, counteracting oxidative damage in varying levels. POD play minor roles in scavenging deleterious LPO, while enhanced SOD, CAT and GST activities in response to thermal stress likely play a more important role against oxidative damage. Here, we firstly identified five LbHsps (four LbHsp70s and one LbHsp110) from psocids, and most of these LbHsps (except LbHsp70-1) are highly expressed at fourth instar nymph and adults, and LbHsp70-1 likely presents as a cognate form of HSP due to its non-significant changes of expression. Most LbHsp70s (except LbHsp70-4) are significantly induced at moderate high temperatures (<40 degrees C) and decreased at extreme high temperatures (40-45 degrees C), but LbHsp110-1 can be significantly induced at all high temperatures. Results of this study suggest that the LbHsp70s and LbHsp110 genes are involved in tolerance to thermal stress in L. bostrychophila, and antioxidant enzymes and heat shock proteins may be coordinately involved in the tolerance to thermal stress in psocids.
引用
收藏
页码:1 / 16
页数:14
相关论文
共 55 条
[1]   Response of antioxidant enzymes in Mythimna separata (Lepidoptera: Noctuidae) exposed to thermal stress [J].
Ali, A. ;
Rashid, M. A. ;
Huang, Q. Y. ;
Wong, C. ;
Lei, C. -L. .
BULLETIN OF ENTOMOLOGICAL RESEARCH, 2017, 107 (03) :382-390
[2]   The mortality of three species of Psocoptera, Liposcelis bostrychophila Badonnel, Liposcelis decolor Pearman and Liposcelis paeta Pearman, at moderately elevated temperatures [J].
Beckett, SJ ;
Morton, R .
JOURNAL OF STORED PRODUCTS RESEARCH, 2003, 39 (01) :103-115
[3]   Heat-shock protein 70 inhibits apoptosis by preventing recruitment of procaspase-9 to the Apaf-1 apoptosome [J].
Beere, HM ;
Wolf, BB ;
Cain, K ;
Mosser, DD ;
Mahboubi, A ;
Kuwana, T ;
Tailor, P ;
Morimoto, RI ;
Cohen, GM ;
Green, DR .
NATURE CELL BIOLOGY, 2000, 2 (08) :469-475
[4]   Plasticity of life-cycle, physiological thermal traits and Hsp70 gene expression in an insect along the ontogeny: Effect of temperature variability [J].
Belen Arias, Maria ;
Josefina Poupin, Maria ;
Lardies, Marco A. .
JOURNAL OF THERMAL BIOLOGY, 2011, 36 (06) :355-362
[5]   Glutathione transferases, regulators of cellular metabolism and physiology [J].
Board, Philip G. ;
Menon, Deepthi .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2013, 1830 (05) :3267-3288
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].
Bustin, Stephen A. ;
Benes, Vladimir ;
Garson, Jeremy A. ;
Hellemans, Jan ;
Huggett, Jim ;
Kubista, Mikael ;
Mueller, Reinhold ;
Nolan, Tania ;
Pfaffl, Michael W. ;
Shipley, Gregory L. ;
Vandesompele, Jo ;
Wittwer, Carl T. .
CLINICAL CHEMISTRY, 2009, 55 (04) :611-622
[8]   Effects of temperature on the activity of antioxidant enzymes in larvae of Bactrocera dorsalis (Diptera: Tephritidae) parasitized by Diachasmimorpha longicaudata (Hymenoptera: Braconidae): Optimizing the mass rearing of this braconid by varying the temperature [J].
Cai, Pumo ;
Wang, Yong ;
Yi, Chuandong ;
Zhang, Qiwen ;
Xia, Huimin ;
Li, Jia ;
Zhang, Hehe ;
Yang, Jianquan ;
Ji, Qinge ;
Chen, Jiahua .
EUROPEAN JOURNAL OF ENTOMOLOGY, 2019, 116 :1-9
[9]   Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress [J].
Chen, Hongsong ;
Solangi, Ghulam Sarwar ;
Guo, Jianying ;
Wan, Fanghao ;
Zhou, Zhongshi .
FRONTIERS IN PHYSIOLOGY, 2018, 9
[10]   Cloning of heat shock protein genes (hsp70, hsc70 and hsp90) and their expression in response to larval diapause and thermal stress in the wheat blossom midge, Sitodiplosis mosellana [J].
Cheng, Weining ;
Li, Dan ;
Wang, Yue ;
Liu, Yang ;
Zhu-Salzman, Keyan .
JOURNAL OF INSECT PHYSIOLOGY, 2016, 95 :66-77