T-cadherin activates Rac1 and Cdc42 and changes endothelial permeability

被引：10

作者：

Semina, E. V. ^{[2
]}

Rubina, K. A. ^{[1
]}

Rutkevich, P. N. ^{[2
]}

Voyno-Yasenetskaya, T. A. ^{[3
]}

Parfyonova, Y. V. ^{[2
]}

Tkachuk, V. A. ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Fundamental Med, Moscow 117192, Russia

[2] Cardiol Res Ctr, Inst Expt Cardiol, Moscow 121552, Russia

[3] Univ Illinois, Dept Pharmacol, Chicago, IL USA

来源：

BIOCHEMISTRY-MOSCOW | 2009年 / 74卷 / 04期

关键词：

T-cadherin; Rho GTPases; endothelial cell permeability; microtubules; actin; CELL-ADHESION; RHO GTPASES; EXPRESSION; POLARIZATION; MIGRATION; THROMBIN; KINASE;

D O I：

10.1134/S0006297909040026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, expression of T-cadherin was shown to induce intracellular signaling in NIH3T3 fibroblasts: it activated Rac1 and Cdc42 (p < 0.01) but not RhoA. T-Cadherin overexpression in human umbilical vein endothelial cells (HUVEC) using adenoviral constructs induced disassembly of microtubules and polymerization of actin stress fibers, whereas down-regulation of endogenous T-cadherin expression in HUVEC using lentiviral constructs resulted in micro-tubule polymerization and a decrease in the number of actin stress fibers. Moreover, suppression of the T-cadherin expression significantly decreased the endothelial monolayer permeability as compared to the control (p < 0.001).

引用

页码：362 / 370

页数：9

共 28 条

[1] Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection [J].

Batard, P ;

Jordan, M ;

Wurm, F .

GENE, 2001, 270 (1-2) :61-68

[2] Microtubule disassembly induces cytoskeletal remodeling and lung vascular barrier dysfunction: Role of Rho-dependent mechanisms [J].

Birukova, AA ;

Smurova, K ;

Birukov, KG ;

Usatyuk, P ;

Liu, F ;

Kaibuchi, K ;

Ricks-Cord, A ;

Natarajan, V ;

Alieva, I ;

Garcia, JGN ;

Verin, AD .

JOURNAL OF CELLULAR PHYSIOLOGY, 2004, 201 (01) :55-70

[3] Rho GTPases and their effector proteins [J].

Bishop, AL ;

Hall, A .

BIOCHEMICAL JOURNAL, 2000, 348 (02) :241-255

[4] Involvement of microtubules, p38, and Rho kinases pathway in 2-methoxyestradiol-induced lung vascular barrier dysfunction [J].

Bogatcheva, Natalia V. ;

Adyshev, Djanybek ;

Mambetsariev, Bolot ;

Moldobaeva, Nurgul ;

Verin, Alexander D. .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2007, 292 (02) :L487-L499

[5] Regulation of cadherin function by Rho and Rac: Modulation by junction maturation and cellular context [J].

Braga, VMM ;

Del Maschio, A ;

Machesky, L ;

Dejana, E .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (01) :9-22

[6] Thrombin inactivates myosin light chain phosphatase via Rho and its target Rho kinase in human endothelial cells [J].

Essler, M ;

Amano, M ;

Kruse, HJ ;

Kaibuchi, K ;

Weber, PC ;

Aepfelbacher, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (34) :21867-21874

[7] Cadherin-mediated cell-cell adhesion and tissue segregation in relation to malignancy [J].

Foty, RA ;

Steinberg, MS .

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2004, 48 (5-6) :397-409

[8]

FREDETTE BJ, 1994, J NEUROSCI, V14, P7331

[9]

Fredette BJ, 1996, DEVELOPMENT, V122, P3163

[10] Cadherin:catenin complex:: A novel regulator of vascular smooth muscle cell behaviour [J].

George, S. J. ;

Beeching, C. A. .

ATHEROSCLEROSIS, 2006, 188 (01) :1-11

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