Molecular Epidemiology of Clinical Cryptococcus neoformans Isolates in Seoul, Korea

被引：19
作者：
Park, So Hae [1 ]
Kim, Mina [2 ]
Joo, Sei Ick [3 ]
Hwang, Soo Myung [1 ]
机构：
[1] Catholic Univ Pusan, Dept Clin Lab Sci, Pusan 609757, South Korea
[2] Univ Ulsan, Dept Lab Med, Sch Med, Seoul 138736, South Korea
[3] Seoul Natl Univ Hosp, Dept Lab Med, Seoul 110744, South Korea
来源：
MYCOBIOLOGY | 2014年 / 42卷 / 01期
关键词：
Cryptococcus neoformans; Molecular type; Multilocus sequencing type; HIV-ASSOCIATED CRYPTOCOCCOSIS; AMPLIFIED POLYMORPHIC DNA; MATING-TYPE; SEROTYPE-A; POPULATION; GENOTYPE; PCR;
D O I：
10.5941/MYCO.2014.42.1.73
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Cryptococcal infection is primarily caused by two species, Cryptococcus neoformans and C. gattii. Between the two species, C. neoformans var. grubii is the major causative agent of cryptococcosis in Asia. We investigated the molecular characteristics of 46 isolates of C. neoformans from patients with cryptococcosis between 2008 and 2012 in Seoul, Korea. All the isolates were determined to be C. neoformans var. grubii (serotype A), mating type MAT alpha, and molecular type VNI by PCR-restriction fragment length polymorphism of the URA5 gene. Multilocus sequencing type (MLST) analysis using the International Society of Human and Animal Mycoses (ISHAM) consensus MLST scheme identified two sequence types (ST). Out of the 46 strains, 44 (95.7%) were identified as ST5, and remaining 2 were identified as ST31. Our study revealed that the clinical strains of C. neoformans in Korea are genetically homogeneous with the VNI/ST5 genotypes, and new appearance of VNI/ST31 genotype may serve as an important indicator of global genetic analysis.
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页码：73 / 78
页数：6
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