Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

被引：39
|
作者：
Levine, Samantha R. [1 ]
Beatty, Kimberly E. [1 ]
机构：
[1] Oregon Hlth & Sci Univ, Dept Biomed Engn, OHSU Ctr Spatial Syst Biomed, 2730 SW Moody Ave,CL3B, Portland, OR 97201 USA
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 09期
关键词：
FLUOROGENIC PROBES; DYES; MICROSCOPY; STRATEGIES; LIPASES;
D O I：
10.1039/c5cc08764c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1'-cyclohexane]-2',5'-diene-2,4'-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as "turn-on'' substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.
引用
收藏
页码：1835 / 1838
页数：4
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