Performance and microbial communities of completely autotrophic denitrification in a bioelectrochemically-assisted constructed wetland system for nitrate removal

被引：98
作者：
Xu, Dan [1 ,2 ]
Xiao, Enrong [2 ]
Xu, Peng [2 ,3 ]
Zhou, Yin [2 ,3 ]
He, Feng [2 ]
Zhou, Qiaohong [2 ]
Xu, Dong [2 ]
Wu, Zhenbin [2 ]
机构：
[1] Wuhan Univ Technol, Coll Resources & Environm Engn, Wuhan 430070, Peoples R China
[2] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
[3] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2017年 / 228卷
基金：
中国国家自然科学基金;
关键词：
Constructed wetland; Autotrophic denitrification; Bioelectrochemical; Microbial community; Open and closed circuit; WASTE-WATER TREATMENT; NITROGEN REMOVAL; DRINKING-WATER; AUTOHYDROGENOTROPHIC DENITRIFICATION; GROUNDWATER; METABOLISM; EFFLUENT; TERMS; RATIO;
D O I：
10.1016/j.biortech.2016.12.065
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A bioelectrochemically-assisted constructed wetland (BECW) system was used to treat nitrate-contaminated wastewater without organic carbon source. The denitrification performance and microbial community composition of a BECW in closed-circuit mode (BECW-C) was compared to a BECW in open-circuit mode (BECW-O). The highest denitrification efficiency (78.92 +/- 3.12%) was obtained in the BECWC with an applied current of 15 mA. No nitrite accumulation was observed during the autotrophic denitrification process in the BECW-C. The significantly higher denitrification efficiency of the BECW-C compared to the BECW-O suggested enhanced denitrification due to in situ generation of hydrogen. The bacterial communities in the anode, cathode and rhizosphere regions collected from the BECW-C (with 10 or 15 mA) and the BECW-O were characterized using 16S rRNA pyrosequencing technology, which revealed different microbial community structures among the treatments. The results also indicated that Thiohalophilus and Clostridium sensu stricto might be responsible for autotrophic denitrification in the BECW-C. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：39 / 46
页数：8
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