Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

被引：121

作者：

Chang, Ki-Young ^{[1
,2
]}

Woo, Doyeon ^{[2
,3
]}

Jung, Hyunjin ^{[2
]}

Lee, Sangkyu ^{[1
,2
]}

Kim, Sungsoo ^{[2
]}

Won, Joungha ^{[2
]}

Kyung, Taeyoon ^{[2
]}

Park, Hyerim ^{[2
]}

Kim, Nury ^{[1
,2
]}

Yang, Hee Won ^{[2
]}

Park, Jae-Yong ^{[3
,4
,5
]}

Hwang, Eun Mi ^{[3
]}

Kim, Daesoo ^{[2
]}

Do Heo, Won ^{[1
,2
]}

机构：

[1] Inst for Basic Sci, Ctr Cognit & Social, Taejon 305701, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

[3] Korea Inst Sci & Technol, WCI Ctr Funct Connect, Seoul 136791, South Korea

[4] Gyeongsang Natl Univ, Sch Med, Inst Hlth Sci, Dept Neurobiol, Jinju 660751, South Korea

[5] Gyeongsang Natl Univ, Sch Med, Med Res Ctr Neural Dysfunct, Jinju 660751, South Korea

来源：

NATURE COMMUNICATIONS | 2014年 / 5卷

基金：

新加坡国家研究基金会;

关键词：

NERVE GROWTH-FACTOR; PROTEIN INTERACTIONS; TRK RECEPTORS; ACTIVATION; INDUCTION; CELLS; TRANSDUCTION; DIFFERENTIATION; PARTNERS; LIGAND;

D O I：

10.1038/ncomms5057

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosin-related kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488 nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.

引用

页数：10

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