Factors Influencing Formation of the Plantlet from Microspore Embryoid in Pakchoi (Brassica Campestris ssp Chinensis L)

被引：0
|
作者：
Liu Xueli [1 ]
Yang Shuo [1 ]
Fu Ying [1 ]
Feng Hui [1 ]
机构：
[1] Shenyang Agr Univ, Coll Hort, Shenyang, Peoples R China
来源：
3RD CONFERENCE ON KEY TECHNOLOGY OF HORTICULTURE, CKTH 2011 | 2011年
关键词：
Pakchoi; microspore culture; embryoid; plantlet regeneration; EMBRYOGENIC ABILITY; CULTURE; CROPS;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
The aim of this study was to optimize culture system for plantlet regeneration from embryoids in Pakchoi. Isolated microspore culture was employed in 10 hybrid varieties. The effects of genotype, seal materials and concentration of different additives to the medium on the formation of plantlet were studied by setting up different treatments. Results indicated that genotypes significantly affected the frequency of plantlet regeneration. The optimum medium was "MS+3%sucrose+1.0%agar+0.1g center dot L(-1)activated charcoal"; Ventilate well Sealed film was beneficial to plantlet regeneration.
引用
收藏
页码：257 / 261
页数：5
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