Comparison of Methods to Distinguish Diploid and Tetraploid Potato in Applied Diploid Breeding

被引:16
作者
Alsahlany, Maher [1 ,2 ]
Zarka, Daniel [1 ]
Coombs, Joseph [1 ]
Douches, David S. [1 ]
机构
[1] Michigan State Univ, Dept Plant Soil & Microbial Sci, 1066 Bogue St, E Lansing, MI 48824 USA
[2] Al Qasim Green Univ, Dept Coll Agr, Hort, Babylon, Iraq
关键词
Potato ploidy level; Chloroplast count; Single nucleotide polymorphism (SNP); Flow cytometry; Diploid potato (2n=2x=24); Tetraploid potato (2n=4x=48); NUCLEAR-DNA CONTENT; DIHAPLOID SOLANUM-TUBEROSUM; FLOW-CYTOMETRIC ANALYSIS; PLOIDY LEVEL; SOMATIC HYBRIDS; SIZE; RESISTANCE; POLYSOMATY; PHUREJA; CROSSES;
D O I
10.1007/s12230-018-09710-7
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Diploid (2n=2x=24) and tetraploid (2n=4x=48) germplasm are commonly used in potato breeding programs. Potato breeders need to efficiently and inexpensively differentiate between diploid and tetraploid progeny in dihaploid induction crosses as well as 2x-2x crosses where 2n gametes may occur in the parents. In this study, we compared the chloroplast count, genome-wide SNP genotyping and flow cytometry methods to determine ploidy. Twenty-eight clones were used as reference samples (14 diploid lines (2n=2x=24), 14 tetraploid (2n=4x=48) varieties and advanced breeding lines) to compare the three ploidy determination methods. The chloroplast count method was used to determine the ploidy level in the 28 reference samples, and 102 potato breeding lines derived from diploid (2x-2x) crosses. The Infinium 12KV2 Potato Single Nucleotide Polymorphism (SNP) Array was used to examine the 28 reference samples and the 102 breeding lines. The results obtained from both chloroplasts counts and SNP genotyping techniques determined that there was a total of 84 diploid lines and 18 tetraploid lines. Flow cytometry was also used to determine ploidy level in a subset of 42 lines (28 reference lines set and 14 breeding lines). All three methods of ploidy determination (chloroplast counting, SNP genotyping and flow cytometry) agreed for all samples evaluated. These results demonstrate the usefulness of chloroplast counting as an efficient and inexpensive method for breeders to differentiate ploidy between diploid and tetraploid potato in applied breeding programs.
引用
收藏
页码:244 / 254
页数:11
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