Green synthesis of gold nanoparticles with starch-glucose and application in bioelectrochemistry

被引:153
作者
Engelbrekt, Christian [1 ]
Sorensen, Karsten H. [1 ]
Zhang, Jingdong [1 ]
Welinder, Anna C. [1 ]
Jensen, Palle S. [1 ]
Ulstrup, Jens [1 ]
机构
[1] Tech Univ Denmark, Dept Chem, NanoDTU, DK-2800 Lyngby, Denmark
关键词
PSEUDOMONAS-AERUGINOSA AZURIN; CYTOCHROME-C; ELECTRON-TRANSFER; ELECTROCHEMISTRY; MONOLAYERS; OXIDATION; VOLTAMMETRY; REDUCTION; SURFACES; BIOLOGY;
D O I
10.1039/b911111e
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A method for gold nanoparticle (AuNP) synthesis from buffered glucose and starch solution has been developed and the particles investigated by UV-Vis spectroscopy, transmission electron microscopy (TEM), atomic force microscopy (AFM) and electrochemistry. The synthesis proceeds smoothly in neutral and basic solution. The starch concentration, temperature and chemical nature of the buffers are key factors in the AuNP formation. Glucose and starch are reducing and protecting agents, respectively. Among several inorganic and biological Good's buffers, phosphate and MES buffers give the best results with quite uniform AuNPs. Other buffers do not result in well-defined nanoparticle structures. Typical AuNP diameters from MES and phosphate buffers (PB) are 4 +/- 1 nm and 13 +/- 2 nm with plasmon band peaks at 521 nm and 523 nm, respectively. The role of the phosphate buffer is mainly to control the pH, while MES is also a synergist with more composite function. AuNPs prepared by this method are stable in solution even after 17 months at room temperature. TEM confirms the crystalline structure of the AuNPs, meaning that the AuNP surfaces are low-index single-crystal facets such as (100), (110) and (111). Electrochemistry of the buffers at such single-crystal gold electrode surfaces has offered a more detailed understanding of the buffer effect. The AuNPs have been successfully used in bioelectrochemistry, and found to efficiently enhance interfacial electrochemical electron transfer of the metalloprotein yeast cytochrome c in homogeneous solution. The synthesis has been extended successfully to direct use of starch-rich foods such as potato, carrot and onion to synthesize AuNPs. The present work thus offers a gentle and non-toxic procedure for the synthesis of monodisperse AuNPs in neutral medium with promising potential for pH sensitive biological or medically related applications.
引用
收藏
页码:7839 / 7847
页数:9
相关论文
共 46 条
[21]   Ordered anion adlayers on metal electrode surfaces [J].
Magnussen, OM .
CHEMICAL REVIEWS, 2002, 102 (03) :679-725
[22]   The use of microorganisms for the formation of metal nanoparticles and their application [J].
Mandal, D ;
Bolander, ME ;
Mukhopadhyay, D ;
Sarkar, G ;
Mukherjee, P .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 69 (05) :485-492
[23]  
Mukherjee P., 2005, CHEMBIOCHEM, V5, P461
[24]   Gold Nanoparticles in Biology: Beyond Toxicity to Cellular Imaging [J].
Murphy, Catherine J. ;
Gole, Anand M. ;
Stone, John W. ;
Sisco, Patrick N. ;
Alkilany, Alaaldin M. ;
Goldsmith, Edie C. ;
Baxter, Sarah C. .
ACCOUNTS OF CHEMICAL RESEARCH, 2008, 41 (12) :1721-1730
[25]   Green nanotechnology from tea: phytochemicals in tea as building blocks for production of biocompatible gold nanoparticles [J].
Nune, Satish K. ;
Chanda, Nripen ;
Shukla, Ravi ;
Katti, Kavita ;
Kulkarni, Rajesh R. ;
Thilakavathy, Subramanian ;
Mekapothula, Swapna ;
Kannan, Raghuraman ;
Katti, Kattesh V. .
JOURNAL OF MATERIALS CHEMISTRY, 2009, 19 (19) :2912-2920
[26]  
PERISTORTAJADA M, 2004, STARCH FOOD STRUCTUR, pCH6
[27]   ELECTROCHIMIE DES SELS DOR .I. ACIDES AURO ET AURICHLORHYDRIQUES ET SELS CORRESPONDANTS [J].
POURADIER, J ;
GADET, MC ;
CHATEAU, H .
JOURNAL DE CHIMIE PHYSIQUE, 1965, 62 (02) :203-+
[28]   Completely "green" synthesis and stabilization of metal nanoparticles [J].
Raveendran, P ;
Fu, J ;
Wallen, SL .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (46) :13940-13941
[29]   Starch content and yield increase as a result of altering adenylate pools in transgenic plants [J].
Regierer, B ;
Fernie, AR ;
Springer, F ;
Perez-Melis, A ;
Leisse, A ;
Koehl, K ;
Willmitzer, L ;
Geigenberger, P ;
Kossmann, J .
NATURE BIOTECHNOLOGY, 2002, 20 (12) :1256-1260
[30]   REDOX REACTION-MECHANISM OF CYTOCHROME-C AT MODIFIED GOLD ELECTRODES [J].
SAGARA, T ;
NIWA, K ;
SONE, A ;
HINNEN, C ;
NIKI, K .
LANGMUIR, 1990, 6 (01) :254-262