Generation of transgenic potato plants highly resistant to potato virus Y (PVY) through RNA silencing

被引：105
作者：
Missiou, A
Kalantidis, K [1 ]
Boutla, A
Tzortzakaki, S
Tabler, M
Tsagris, M
机构：
[1] Fdn Res & Technol Hellas, Inst Mol Biol & Biotechnol, Iraklion, Crete, Greece
[2] Univ Crete, Dept Biol, Iraklion 71110, Crete, Greece
来源：
MOLECULAR BREEDING | 2004年 / 14卷 / 02期
关键词：
coat protein gene; PTGS; PVY; RNA silencing; siRNA; Solanum tuberosum; transgenic plants;
D O I：
10.1023/B:MOLB.0000038006.32812.52
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
In this study we applied RNA silencing to engineer potato plants that are resistant to potato virus Y (PVY). We expressed double-stranded (ds) RNA derived from the 3' terminal part of the coat protein gene of PVY, which is highly conserved in sequence amongst different PVY isolates, in transgenic potatoes of the commercial variety 'Spunta'. Transgenic plants were analyzed for generation of transgene-derived short interfering RNAs (siRNAs) prior to virus inoculation. Twelve of fifteen transgenic lines produced siRNAs and were highly resistant to three strains of PVY, each belonging to three different subtypes of the virus (PVYN, PVYO and PVYNTN). Infection of transgenic plants with Potato virus X (PVX) simultaneously or prior to the challenge with PVY did not interfere with PVY-resistance.
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页码：185 / 197
页数：13
相关论文
共 52 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   A viral suppressor of gene silencing in plants [J].
Anandalakshmi, R ;
Pruss, GJ ;
Ge, X ;
Marathe, R ;
Mallory, AC ;
Smith, TH ;
Vance, VB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (22) :13079-13084
[3]   RNA silencing - Diced defence [J].
Baulcombe, D .
NATURE, 2001, 409 (6818) :295-296
[4]   Agrobacterium-mediated transformation of three economically important potato cultivars using sliced internodal explants:: an efficient protocol of transformation [J].
Beaujean, A ;
Sangwan, RS ;
Lecardonnel, A ;
Sangwan-Norreel, BS .
JOURNAL OF EXPERIMENTAL BOTANY, 1998, 49 (326) :1589-1595
[5]   Role for a bidentate ribonuclease in the initiation step of RNA interference [J].
Bernstein, E ;
Caudy, AA ;
Hammond, SM ;
Hannon, GJ .
NATURE, 2001, 409 (6818) :363-366
[6]   RETRACTED: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana (Retracted article. See vol. 34, pg. 2595, 2015) [J].
Brigneti, G ;
Voinnet, O ;
Li, WX ;
Ji, LH ;
Ding, SW ;
Baulcombe, DC .
EMBO JOURNAL, 1998, 17 (22) :6739-6746
[7]   SUBCELLULAR-LOCALIZATION OF THE 25-KDA PROTEIN ENCODED IN THE TRIPLE GENE BLOCK OF POTATO VIRUS-X [J].
DAVIES, C ;
HILLS, G ;
BAULCOMBE, DC .
VIROLOGY, 1993, 197 (01) :166-175
[8]   BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].
DITTA, G ;
STANFIELD, S ;
CORBIN, D ;
HELINSKI, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351
[9]  
DOUGHERTY WG, 1994, MOL PLANT MICROBE IN, V7, P544, DOI 10.1094/MPMI-7-0554
[10]   TRANSGENES AND GENE SUPPRESSION - TELLING US SOMETHING NEW [J].
DOUGHERTY, WG ;
PARKS, TD .
CURRENT OPINION IN CELL BIOLOGY, 1995, 7 (03) :399-405
123456