Comprehensive analysis of Huanglian Jiedu decoction: Revealing the presence of a self-assembled phytochemical complex in its naturally-occurring precipitate

被引：33
作者：
Chen, Meng [1 ,2 ]
Wang, Penglong [2 ]
Li, Tong [2 ]
Li, Linsen [1 ,3 ]
Li, Junfang [1 ]
Bai, Hua [1 ]
Lei, Haimin [2 ]
Ma, Qiang [1 ]
机构：
[1] Chinese Acad Inspect & Quarantine, Beijing 100176, Peoples R China
[2] Beijing Univ Chinese Med, Sch Chinese Mat Med, Beijing 102488, Peoples R China
[3] Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2021年 / 195卷
基金：
中国国家自然科学基金;
关键词：
Huanglian Jiedu decoction; Naturally-occurring precipitate; Ultra-high-performance liquid chromatography; Quadrupole-Orbitrap high-resolution mass spectrometry; Fragmentation pathway; Self-assembly;
D O I：
10.1016/j.jpba.2020.113820
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The current study presents a comprehensive analysis to explore the compositions of both the supernatant and naturally-occurring precipitate of Huanglian Jiedu decoction employing ultra-high-performance liquid chromatography hyphenated with quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). Totally 109 constituents (32 alkaloids, 39 flavonoids, 12 iridoids, 9 phenolic acids, and 17 other compounds) were identified from accurate-mass measurements in full-scan MS/data-dependent MS/MS mode of acquisition. Furthermore, a quantitative method was developed for determination of 14 marker compounds in Huanglian Jiedu decoction. Experimental results revealed that all of these marker compounds were present in both the supernatant and naturally-occurring precipitate. Most notably, the contents of baicalin and berberine were significantly higher in the naturally-occurring precipitate than supernatant, presumably due to self-assembly complexation. The formation of the baicalin/berberine complex was comprehensively investigated by electrospray ionization (ESI)-MS, nuclear magnetic resonance (NMR), ultraviolet-visible (UV-vis), Fourier transform infrared (FTIR), and fluorescence spectroscopy, etc. The morphology and size distribution of the baicalin/berberine self-assembled nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). This study provides fundamental scientific evidence of the presence of a self-assembled phytochemical complex in the naturally-occurring precipitate, enabling better understanding of Huanglian Jiedu decoction. (C) 2020 Elsevier B.V. All rights reserved.
引用
收藏
页数：11
相关论文
共 32 条
[1]  
Zhang A., Sun H., Han Y., Wang X., Chapter 4 - UPLC/MS and its potential in traditional Chinese medicine development, Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications, pp. 23-35, (2017)
[2]  
Yang M., Tao S., Guan S., Wu X., Xu P., Guo D., 3.13 - Chinese traditional medicine, Comprehensive Natural Products II: Chemistry and Biology, pp. 383-477, (2010)
[3]  
Yan S., Zhao J., Nagle D.G., Zhou Y., Zhang W., Chapter 1 - strategy for modern research of traditional Chinese medicine formulae, Systems Biology and its Application in TCM Formulas Research, pp. 3-18, (2018)
[4]  
Wang X., Zhang A., Sun H., Yan G., Wang P., Han Y., Chapter 1 - traditional Chinese medicine: current state, challenges, and applications, Serum Pharmacochemistry of Traditional Chinese Medicine: Technologies, Strategies and Applications, pp. 1-6, (2017)
[5]  
Qi Y., Zhang Q., Zhu H., Huang-Lian Jie-Du decoction: a review on phytochemical, pharmacological and pharmacokinetic investigations, Chin. Med., 14, 1, (2019)
[6]  
Lin L.-T., Wu S.-J., Lin C.-C., The anticancer properties and apoptosis-inducing mechanisms of cinnamaldehyde and the herbal prescription Huang-Lian-Jie-Du-Tang (Huáng Lián Jiě Dú Tang) in human hepatoma cells, J. Tradit. Complementary Med., 3, 4, pp. 227-233, (2013)
[7]  
Zhang H., Fu P., Ke B., Wang S., Li M., Han L., Peng C., Zhang W., Liu R., Metabolomic analysis of biochemical changes in the plasma and urine of collagen-induced arthritis in rats after treatment with Huang-Lian-Jie-Du-Tang, J. Ethnopharmacol., 154, 1, pp. 55-64, (2014)
[8]  
Ma S., Feng C., Dai G., Song Y., Zhou G., Zhang X., Miao C., Yu H., Ju W., In silico target fishing for the potential bioactive components contained in Huanglian Jiedu Tang (HLJDD) and elucidating molecular mechanisms for the treatment of sepsis, Chin. J. Nat. Med., 13, 1, pp. 30-40, (2015)
[9]  
Liao S., Li P., Wang J., Zhang Q., Xu D., Lv Y., Yang M., Kong L., Huang-Lian-Jie-Du decoction treated sepsis via regulating ERK and SRC/STAT3 pathways and ameliorating metabolic status, RSC Adv., 6, 92, pp. 89855-89866, (2016)
[10]  
Wei D., Liao S., Wang J., Yang M., Kong L., Cholestatic liver injury model of bile duct ligation and the protection of Huang-Lian-Jie-Du decoction by NMR metabolomic profiling, RSC Adv., 5, 81, pp. 66200-66211, (2015)
1234