Over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase in Klebsiella pneumoniae and their effects on conversion of glycerol into 1,3-propanediol in resting cell system

被引：17

作者：

Zhao, Li ^{[1
]}

Ma, Xingyuan ^{[1
]}

Zheng, Yu ^{[1
]}

Zhang, Jianguo ^{[1
]}

Wei, Guodong ^{[1
]}

Wei, Dongzhi ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 2009年 / 84卷 / 04期

关键词：

1,3-propanediol; 1,3-propanediol oxidoreductase; glycerol dehydrogenase; resting cell system; Klebsiella pneumoniae; ANAEROBIC CONTINUOUS-CULTURE; MICRO-AEROBIC CONDITIONS; CITROBACTER-FREUNDII; FERMENTATION; DISSIMILATION; METABOLISM; 3-HYDROXYPROPIONALDEHYDE; OVEREXPRESSION; ACCUMULATION; PATHWAY;

D O I：

10.1002/jctb.2092

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

BACKGROUND: Glycerol dehydrogenase [EC.1.1.1.6] and 1,3-propanediol oxidoreductase [EC.1.1.1.202] were proved to be two of the key enzymes for glycerol conversion to 1,3-propanediol in Klebsiella pneumoniae under anaerobic conditions. For insight into their significance on 1,3-propanediol production under micro-aerobic conditions, these two enzymes were over-expressed in K. pneumoniae individually, and their effects on conversion of glycerol into 1,3-propanediol in a resting cell system under micro-aerobic conditions were investigated. RESULTS: In the resting cell system, over-expression of 1,3-propanediol oxidoreductase led to faster glycerol conversion and 1,3-propanediol production. After a 12 h conversion process, it improved the yield of 1,3-propanediol by 20.4% (222.1 mmol L-1 versus 184.4 mmol L-1) and enhanced the conversion ratio of glycerol into 1,3-propanediol from 50.8% to 59.8% (mol mol(-1)). Over-expression of glycerol dehydrogenase in K. pneumoniae had no significant influence both on 1,3-propanediol yield and on the conversion ratio of glycerol into 1,3-propanediol in the resting cell system. CONCLUSION: The results were important for an understanding of the significance of glycerol dehydrogenase and 1,3-propanediol oxidoreductase in 1,3-proanediol production under micro-aerobic conditions, and for developing better strategies to improve 1,3-propanediol yield. (c) 2008 Society of Chemical Industry

引用

页码：626 / 632

页数：7

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