Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of spotted maigre (Nibea albiflora)

被引:14
|
作者
Xing, Shichao [1 ,2 ]
Shao, Changwei [1 ]
Liao, Xiaolin [1 ]
Tian, Yongsheng [1 ]
Chen, Songlin [1 ]
机构
[1] Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
[2] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
关键词
Spotted maigre; Nibea albiflora; Microsatellite; Enriched genomic library; EST-LIBRARY;
D O I
10.1007/s10592-008-9663-5
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
In the present study, we reported 13 polymorphic microsatellite loci isolated from a dinucleotide-enriched genomic library of Nibea albiflora. The number of alleles, observed and expected heterozygosity per locus in 44 individuals ranged from 2 to 13, from 0.0909 to 0.9773 and from 0.0886 to 0.9073, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. Analysis of linkage disequilibrium showed non-significant among the two pairs of loci. As a result, 13 microsatellite loci probably located on different chromosome pairs and these polymorphic microsatellite loci should provide sufficient level of genetic diversity to investigate the fine-scale population structure and evaluate the breeding strategy in Nibea albiflora.
引用
收藏
页码:789 / 791
页数:3
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