Synergistic Antitumoral Effect of IL-12 Gene Cotransfected With Antiangiogenic Genes for Angiostatin, Endostatin, and Saxatilin

被引：6

作者：

Kim, Hong Sung ^{[1
]}

Jeong, Hwa Yeon ^{[2
]}

Lee, Yeon Kyung ^{[2
]}

Kim, Keun Sik ^{[3
]}

Park, Yong Serk ^{[2
]}

机构：

[1] Korea Nazarene Univ, Dept Biomed Lab Sci, Cheonan, South Korea

[2] Yonsei Univ, Dept Biomed Lab Sci, Wonju 220710, Gangwon, South Korea

[3] Konyang Univ, Dept Biomed Lab Sci, Taejon, South Korea

来源：

ONCOLOGY RESEARCH | 2013年 / 21卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

IL-12; Angiostatin; Endostatin; Saxatilin; O; O '-Dimyristyl-N-lysyl glutamate (DMKE) cationic liposomes; Combinatorial gene therapy; TUMOR PROGRESSION; THERAPY; CANCER; ANGIOGENESIS; INHIBITION; INTERLEUKIN-12; EXPRESSION; K1-3; COMBINATION; TOXICITY;

D O I：

10.3727/096504014X13907540404798

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Previously, it was reported that the cotransfection of angiostatin K1-3, endostatin, and saxatilin genes using cationic liposomes significantly inhibited tumor progression. IL-12 is a well-known immune modulator that promotes Thl-type antitumor immune responses and also induces antiangiogenic effects. In this study, we have examined the antitumoral function of the IL-12 gene cotransfected with antiangiogenic genes for angiostatin K1-3, endostatin, and saxatilin by O,O '-dimyristyl-N-lysyl glutamate (DMKE) cationic liposomes in a mouse tumor model. According to our results, the administration of the IL-12 gene or the genes for angiostatin K1-3, endostatin, and saxatilin exhibited effective inhibition of B16BL6 melanoma growth in mice. In particular, intravenous administration of the IL-12 gene along with intratumoral administration of the three antiangiogenic genes synergistically inhibited the B16BL6 tumor growth. These results suggest that systemically expressed IL-12 enhances antitumoral efficacy of locally expressed antiangiogenic proteins.

引用

页码：209 / 216

页数：8

共 30 条

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