Activity and stability of caffeine demethylases found in Pseudomonas putida IF-3

被引：22

作者：

Beltran, Juan G. ^{[1
]}

Leask, Richard L. ^{[1
]}

Brown, Wayne A. ^{[1
]}

机构：

[1] McGill Univ, Dept Chem Engn, Montreal, PQ, Canada

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2006年 / 31卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

caffeine; decaffeination; enzymes; enzyme activity; enzyme deactivation; cell disruption;

D O I：

10.1016/j.bej.2006.05.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Resting cell suspensions and cell-free extracts of Pseudomona sputida IF-3 were tested to assess their ability to degrade caffeine, and to determine their capacity to retain activity at different temperatures. A method to quantify cell lysis using optical density was developed in order to allow the comparison of cell free extract and resting cell caffeine degradation rates on the same basis. Caffeine degradation rates for cell free extracts were found to be 2.4 mu mol g cells(-1) min(-1); this rate is 55 times faster than previously reported P. putida data. Resting cells degraded caffeine 12 times faster than cell free extracts, at 22 degrees C. However, both systems were equivalently active at 50 degrees C. Resting cells were significantly more stable than cell free extracts, retaining their ability to degrade caffeine even at elevated temperatures. Cell free extracts lost all activity after 15 min at 55 degrees C. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：8 / 13

页数：6

共 28 条

[1] MICROBIAL-PRODUCTION OF THEOBROMINE FROM CAFFEINE [J].