Voltammetry of osmium-modified DNA at a mercury film electrode application in detecting DNA hybridization

被引：26

作者：

Kostecka, P ^{[1
]}

Havran, L ^{[1
]}

Pivonkova, H ^{[1
]}

Fojta, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, Biophys Chem Lab, CS-61265 Brno, Czech Republic

来源：

BIOELECTROCHEMISTRY | 2004年 / 63卷 / 1-2期

关键词：

osmium; DNA hybridization; mercury film electrode;

D O I：

10.1016/j.bioelechem.2003.11.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2' -bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmiurn signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：245 / 248

页数：4

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