Desulfurization of dibenzothiophene by Bacillus subtilis recombinants carrying dszABC and dszD genes

被引：25
作者：
Ma, Ting
Li, Guoqiang
Li, Jian
Liang, Fenglai
Liu, Rulin [1 ]
机构：
[1] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China
[2] Nankai Univ, Coll Environm Sci & Technol, Tianjin 300071, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 14期
基金：
中国博士后科学基金;
关键词：
Bacillus subtilis; desulfurization; dibenzothiophene; dsz genes; Rhodococcus erythropolis;
D O I：
10.1007/s10529-006-9056-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The desulfurization (dsz) genes from Rhodococcus erythropolis DS-3 were successfully integrated into the chromosomes of Bacillus subtilis ATCC 21332 and UV1 using an integration vector pDGSDN, yielding two recombinant strains, B. subtilis M29 and M28 in which the integrated dsz genes were expressed efficiently under the promoter, Pspac. The dibenzothiophene (DBT) desulfurization efficiency of M29 was 16.2 mg DBT l(-1) h(-1) at 36 h, significantly higher than that of R. erythropolis DS-3 and B. subtilis M28 and also showed no product inhibition. The interfacial tension of the supernatant fermented by M29 varied from 48 mN m(-1) to 4.2 mN m(-1), lower than that of the recombinant strain, M28, reveals that the biosurfactant secreted from M29 may have an important function in the DBT desulfurization process.
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收藏
页码：1095 / 1100
页数：6
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