Electrochemical immunoassay moving into the fast lane

被引:73
作者
Ronkainen-Matsuno, NJ [1 ]
Thomas, JH [1 ]
Halsall, HB [1 ]
Heinemann, WR [1 ]
机构
[1] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA
关键词
electrochemical immunoassay; sandwich immunoassay; alkaline phosphatase; micro total analysis systems;
D O I
10.1016/S0165-9936(02)00401-6
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The evolution of electrochemical immunoassay has resulted in significant improvements in assay times and detection limits. These developments are traced using a sandwich enzyme immunoassay for IgG as representative. Decreasing the volume of the immunoassay reaction chamber, improving electrochemical detection techniques, and reducing non-specific adsorption have been important factors. (C) 2002 Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:213 / 225
页数:13
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