Tcc1p, a novel protein containing the tetratricopeptide repeat motif, interacts with Tup1p to regulate morphological transition and virulence in Candida albicans

被引:20
|
作者
Kaneko, Aki [1 ]
Umeyama, Takashi [1 ]
Utena-Abe, Yuki [1 ]
Yamagoe, Satoshi [1 ]
Niimi, Masakazu [1 ]
Uehara, Yoshimasa [1 ]
机构
[1] Natl Inst Infect Dis, Dept Bioact Mol, Shinjuku Ku, Tokyo 1628640, Japan
关键词
D O I
10.1128/EC.00151-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transcriptional factor CaTup1p represses many genes involved in intracellular processes, including the yeast-hypha transition, in the human fungal pathogen Candida albicans. Using tandem affinity purification technology, we identified a novel protein that interacts with CaTup1p, named Tcc1p (Tup1p complex component). Tcc1p is a C. albicans-specific protein with a 736-amino-acid polypeptide with four tetratricopeptide repeat (TPR) motifs in the N-terminal portion. Tcc1p formed a protein complex with CaTup1p via the TPR domain of Tcc1p, independently of CaSsn6p-CaTup1p. The tcc1 Delta disruptant showed filamentous growth under conditions inducing the yeast form, as is true of the Catup1 Delta mutant. Consistent with this result, the common set of hypha-specific genes was negatively regulated by both TCC1 and CaTUP1. These observations will provide new insights into CaTup1p-dependent transcriptional gene regulation in C. albicans.
引用
收藏
页码:1894 / 1905
页数:12
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