Different LED Light Intensities and 6-Benzyladenine Concentrations in Relation to Shoot Development, Leaf Architecture, and Photosynthetic Pigments of Gerbera jamesonii Bolus In Vitro

A mixture of red and blue light-emitting diodes (LEDs; at a ratio of 7:3, respectively) were used to analyze the effects of different photosynthetic photon flux densities (PPFDs) (40, 80, and 120 mu mol m(-2) s(-1) hereafter known as LED 40, 80, and 120, respectively) on the micropropagation of Gerbera jamesonii Bolus shoots. The experiment also examined the effect of 6-benzyladenine (BA) in 1, 2.5, and 5 mu M concentrations in the media. Biometrical observations and analyses of leaf morphometry and photosynthetic pigment content were conducted. Shoot multiplication increased with an increasing BA concentration. A PPFD of 80 mu mol m(-2) s(-1) and 5 mu M BA is suggested as efficient for shoot propagation and economically viable. LED 120 increased the leaf blade area and its width, and circularity and elongation ratios. The intensity of light did not affect the fresh weight, which increased at higher BA concentrations (2.5 and 5 mu M). The dry weight content decreased with increasing cytokinin concentration; the greatest content was observed on media with 1 mu M BA under PPFD 120 mu mol m(-2) s(-1). LED 80 increased the photosynthetic pigments content in the leaves in comparison to the standard intensity of LED 40. Increased BA concentration raises the content of chlorophyll a.