Identification and microsatellite markers of a resistance gene to powdery mildew in common wheat introgressed from Triticum durum

被引：0
作者：
Zhu, ZD [1 ]
Kong, XY [1 ]
Zhou, RH [1 ]
Jia, JZ [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Crops Germplasm Resources, Minist Agr, Key Lab Crop Germplasm & Biotechnol, Beijing 100081, Peoples R China
来源：
ACTA BOTANICA SINICA | 2004年 / 46卷 / 07期
关键词：
resistance gene; microsatellite; molecular tagging; powdery mildew; durum wheat;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A powdery mildew resistance gene in a BC3F2 population, derived from a cross made between an amphidiploid of Triticum durum Desf.-Aegilops caudata L. and T. aestivum L. cv. Laizhou 953, was identified. Genetic analysis of resistance to powdery mildew in BC3F2 population and derived BC3F3 families indicated a single dominant gene controlled the resistance. By bulk segregation analysis, two microsatellite markers, Xgwm311 and Xgwm382, were identified to be closely linked to the resistance gene with genetic distance of 5.9 cM and 4.9 cM, respectively. DNA from T. durum accession DR147, Ae. caudata ace. Ae14, and recurrent parent wheat (Triticum aestivum L.) cv. Laizhou 953 were amplified with primer pairs WMS311 and WMS382, the specific bands related to the resistance gene were only present in T, durum ace. DR147. Results showed that the resistance gene originated in T. durum ace. DR147. Based on the location of the linked microsatellite markers, the resistance gene was located on the telomeric region of chromosome 2AL in wheat. Temporarily, the resistance gene was designated as PmDR147 The relation of this gene and Pm4 was discussed.
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页码：867 / 872
页数：6
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