Influence of the hollow-fibre membrane on the stability of β-galactosidase and on lactose hydrolysis -: Kinetic models including adsorption of the enzyme onto the membrane

被引：10
|
作者：
Jurado, E. [1 ]
Camacho, F. [1 ]
Luzon, G. [1 ]
Vicaria, J. M. [1 ]
机构：
[1] Univ Granada, Fac Ciencias, Dept Ingn Quim, E-18071 Granada, Spain
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 2006年 / 39卷 / 05期
关键词：
kinetic model; enzymatic stability; membrane adsorption; hollow-fibre bioreactor; lactose hydrolysis;
D O I：
10.1016/j.enzmictec.2006.02.009
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An adsorption-kinetic model has been proposed to explain beta-galactosidase stability in a recirculation hollow-fibre bioreactor at different temperature. The kinetic constants of the model and its dependence on temperature have been evaluated. In addition, a study has been made of the influence of lactose and enzyme concentration on lactose hydrolysis with hollow-fibre modules made of Cuprophan and Polysulfone. The membrane surface areas assayed were between 1 and 1.95 m(2). The experimental results have been satisfactorily explained with the model proposed in a previous work. This model included the competitive inhibition of the enzyme by galactose and the enzymatic adsorption onto the membrane. The adsorption constants have been calculated, verifying the applicability of the model in the modules used. Within the experimental range analysed, the hollow-fibre module made of Polysulfone provided the least adsorption of the enzyme and therefore was the most suitable for this enzyme and reaction. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：1008 / 1015
页数：8
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