Benzaldehyde lyase (BAL)-catalyzed enantioselective C-C bond formation in deep-eutectic-solvents-buffer mixtures

被引：45

作者：

Maugeri, Zaira ^{[1
]}

Dominguez de Maria, Pablo ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, ITMC, D-52074 Aachen, Germany

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2014年 / 107卷

关键词：

Lyases; Deep-eutectic-solvents; Biocatalysis; Non-conventional media; IONIC LIQUIDS; THIAMIN DIPHOSPHATE; CHOLINE-CHLORIDE; BIOTRANSFORMATIONS; SEPARATION; GLYCEROL;

D O I：

10.1016/j.molcatb.2014.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Deep-eutectic-solvents (DES) have emerged in the last decades as promising bio-based and biodegradable neoteric solvents for biocatalysis, with examples covering different enzymes (mostly hydrolases) and whole-cells (baker's yeast). This paper explores for the first time the use of benzaldehyde lyase (BAL), a thiamine-diphosphate dependent lyase (ThDP-Iyase) able to catalyze the carboligation of aldehydes (C-C bond formation) in different DES-buffer mixtures. By using choline chloride-glycerol DES, BAL remains fully active with excellent enantioselectivity at 60:40 DES-buffer (v/v), whereby a significant denaturation is observed at 70:30 mixtures. Remarkably, the use of choline chloride-urea DES as reaction media leads to full conversions with BAL at such solvent-buffer proportions, suggesting that the design of both the biocatalyst and the neoteric solvent may provide useful novel reactive systems for biocatalysis in non-conventional media. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：120 / 123

页数：4

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