Selective antibacterial activity of a novel lactotransferrin-derived antimicrobial peptide LF-1 against Streptococcus mutans

被引：9
作者：
Feng, Zening [1 ,2 ]
Luo, Junyuan [1 ,2 ]
Lyu, Xiaohui [1 ,2 ]
Chen, Yue [1 ,2 ]
Zhang, Linglin [1 ,2 ,3 ]
机构：
[1] Sichuan Univ, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, Chengdu, Peoples R China
[2] Sichuan Univ, West China Hosp Stomatol, Dept Cariol & Endodont, Chengdu, Peoples R China
[3] Sichuan Univ, Natl Clin Res Ctr Oral Dis, State Key Lab Oral Dis, 14,Sect 3 Renmin Rd South, Chengdu 610041, Peoples R China
来源：
ARCHIVES OF ORAL BIOLOGY | 2022年 / 139卷
基金：
中国国家自然科学基金;
关键词：
Antimicrobial peptide; Dental caries; Streptococcus mutans; Lactotransferrin; Membrane disruption; VIRULENCE; CARIES; SITE;
D O I：
10.1016/j.archoralbio.2022.105446
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Objective: Streptococcus mutans is a key pathogen involved in the development of caries lesions. Previously, we developed a novel lactotransferrin-derived antimicrobial peptide LF-1 with potential selective activity against S. mutans. This study aimed to further confirm the selectivity of LF-1 by investigating its effect on S. mutans membrane. Design: The effects of LF-1 on the viability of human gingival fibroblasts (HGFs) and three common oral Streptococcus (S. mutans, S. sanguinis, and S. gordonii) were evaluated and its structural characteristics were analysed using eukaryotic and prokaryotic membrane-simulated liposomes. Membrane affinity of LF-1 to the three streptococci strains was evaluated using the 3',3'-dipropylthiadicarbocyanine iodide experiment, hydrophobicity assay, and flow cytometry analysis. Transmission electron microscopy (TEM) was used to observe morphological changes in the three streptococcal membranes after LF-1 treatment. Results: LF-1 displayed lower cytotoxicity to HGFs and selective antibacterial activity against S. mutans. LF-1 exhibited a typical alpha-helix structure and showed a tryptophan fluorescence blue shift in the prokaryotic membrane-simulated model. The most notable LF-1 induced changes occurred in the membrane potential and hydrophobicity of S. mutans among the three streptococci strains. Furthermore, the fluorescence of fluorescein isothiocyanate-labelled LF-1 was higher in S. mutans than in the other species. TEM showed that 16 mu mol/L LF-1 could induce mesosome-like structures in S. mutans, whereas no significant morphological changes occurred in the other species. Conclusion: LF-1 has selective affinity for and antibacterial activity against S. mutans with strong membrane disrupting ability, highlighting the potential of LF-1 as a crucial antibacterial agent in caries prevention.
引用
收藏
页数：8
相关论文
共 44 条
[41]   Fusion between fluid liposomes and intact bacteria: study of driving parameters and in vitro bactericidal efficacy [J].
Wang, Zhao ;
Ma, Yufan ;
Khalil, Hayssam ;
Wang, Rutao ;
Lu, Tingli ;
Zhao, Wen ;
Zhang, Yang ;
Chen, Jamin ;
Chen, Tao .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2016, 11 :4025-4036
[42]   An alpha/beta chimeric peptide molecular brush for eradicating MRSA biofilms and persister cells to mitigate antimicrobial resistance [J].
Xiao, Ximian ;
Zhang, Si ;
Chen, Sheng ;
Qian, Yuxin ;
Xie, Jiayang ;
Cong, Zihao ;
Zhang, Danfeng ;
Zou, Jingcheng ;
Zhang, Wenjing ;
Ji, Zhemin ;
Cui, Ruxin ;
Qiao, Zhongqian ;
Jiang, Weinan ;
Dai, Yidong ;
Wang, Yun ;
Shao, Xiaoyan ;
Sun, Yun ;
Xia, Jiang ;
Fei, Jian ;
Liu, Runhui .
BIOMATERIALS SCIENCE, 2020, 8 (24) :6883-6889
[43]   Small Molecule Compounds, A Novel Strategy against Streptococcus mutans [J].
Yang, Sirui ;
Zhang, Jin ;
Yang, Ran ;
Xu, Xin .
PATHOGENS, 2021, 10 (12)
[44]   Dual Mode of Anti-Biofilm Action of G3 against Streptococcus mutans [J].
Zhang, Jiangyu ;
Chen, Cuixia ;
Chen, Jiaxi ;
Zhou, Shasha ;
Zhao, Yurong ;
Xu, Minglu ;
Xu, Hai .
ACS APPLIED MATERIALS & INTERFACES, 2020, 12 (25) :27866-27875
12345