Alginate molecular mass produced by Azotobacter vinelandii in response to changes of the O2 transfer rate in chemostat cultures

被引：12

作者：

Diaz-Barrera, A. ^{[1
]}

Silva, P. ^{[1
]}

Avalos, R. ^{[1
]}

Acevedo, F. ^{[1
]}

机构：

[1] Pontificia Univ Catolica Valparaiso, Escuela Ingn Bioquim, Valparaiso 4059, Chile

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 06期

关键词：

Alginate; Chemostat; Molecular mass; Oxygen transfer rate; OXYGEN-TRANSFER RATE; PSEUDOMONAS-AERUGINOSA; WEIGHT; BIOSYNTHESIS;

D O I：

10.1007/s10529-009-9949-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O-2 transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g l(-1)) was observed. At a constant growth rate (0.1 h(-1)), the mean molecular mass of the alginate was strongly influenced by changes in the OTR, varying from 860 to 1,690 kDa. These results support a possible relationship between alginate polymerization-depolymerization process and the O-2 uptake rate.

引用

页码：825 / 829

页数：5

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