Preparation of Robust Biocatalyst Based on Cross-Linked Enzyme Aggregates Entrapped in Three-Dimensionally Ordered Macroporous Silica

被引：80
作者：
Jiang, Yanjun [1 ]
Shi, Lianlian [1 ]
Huang, Yan [1 ]
Gao, Jing [1 ]
Zhang, Xu [1 ]
Zhou, Liya [1 ]
机构：
[1] Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin, Peoples R China
来源：
ACS APPLIED MATERIALS & INTERFACES | 2014年 / 6卷 / 04期
关键词：
Three-dimensionally ordered macroporous materials; cross-linked enzyme aggregates; lipase; enzyme immobilization; biodiesel; SEPARABLE MESOPOROUS SILICA; CATALYTIC-PROPERTIES; BIODIESEL PRODUCTION; ORGANIC-SOLVENT; ALPHA-AMYLASE; IMMOBILIZATION; LIPASE; CLEAS; STABILIZATION; PERFORMANCE;
D O I：
10.1021/am405104b
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
With the aim to provide a highly stable and active biocatalyst, cross-linked enzyme aggregates (CLEAs) of lipase Candida sp. 99-125 were prepared in three-dimensionally ordered macroporous silica materials (CLEAs-LP@ 3DOM-SiO2). Lipase Candida sp. 99-125 was first precipitated in the pores of 3DOM SiO2 (named EAs-LP@3DOM-SiO2), and further cross-linked by glutaraldehyde to form CLEAs-LP@3DOM-SiO2. Saturated ammonium sulfate was used as a precipitant and glutaraldehyde with a concentration of 0.25% (w/w) was employed as a cross-linker. Compared with EAs-LP@ 3DOM-SiO2 and native lipase, CLEAs-LP@3DOM-SiO2 exhibited excellent thermal and mechanical stability, and could maintain more than 85% of initial activity after 16 days of shaking in organic and aqueous phase. When CLEAs-LP@3DOM-SiO2 was applied in esterification and transesterification reactions, improved activity and reusability were achieved. This method can be used for the immobilization of other enzymes of interest.
引用
收藏
页码：2622 / 2628
页数：7
相关论文
共 44 条
[1]   Immobilisation and application of lipases in organic media [J].
Adlercreutz, Patrick .
CHEMICAL SOCIETY REVIEWS, 2013, 42 (15) :6406-6436
[2]   Functionalization of mesoporous silica for lipase immobilization -: Characterization of the support and the catalysts [J].
Blanco, RM ;
Terreros, P ;
Fernández-Pérez, M ;
Otero, C ;
Díaz-González, G .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 30 (02) :83-93
[3]   Controlling performance of lipase immobilised on bioinspired silica [J].
Forsyth, Claire ;
Patwardhan, Siddharth V. .
JOURNAL OF MATERIALS CHEMISTRY B, 2013, 1 (08) :1164-1174
[4]   Formation of lipase Candida sp 99-125 CLEAs in mesoporous silica: characterization and catalytic properties [J].
Gao, Jing ;
Shi, Lianlian ;
Jiang, Yanjun ;
Zhou, Liya ;
He, Ying .
CATALYSIS SCIENCE & TECHNOLOGY, 2013, 3 (12) :3353-3359
[5]   Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance [J].
Garcia-Galan, Cristina ;
Berenguer-Murcia, Angel ;
Fernandez-Lafuente, Roberto ;
Rodrigues, Rafael C. .
ADVANCED SYNTHESIS & CATALYSIS, 2011, 353 (16) :2885-2904
[6]   Reactive extraction and in situ self-catalyzed methanolysis of germinated oilseed for biodiesel production [J].
Gu, Hanqi ;
Jiang, Yanjun ;
Zhou, Liya ;
Gao, Jing .
ENERGY & ENVIRONMENTAL SCIENCE, 2011, 4 (04) :1337-1344
[7]   Understanding enzyme immobilisation [J].
Hanefeld, Ulf ;
Gardossi, Lucia ;
Magner, Edmond .
CHEMICAL SOCIETY REVIEWS, 2009, 38 (02) :453-468
[8]   Preparation and characterization of cross-linked enzyme aggregates (CLEAs) of recombinant poly-3-hydroxybutyrate depolymerase from Streptomyces exfoliatus [J].
Hormigo, Daniel ;
Garcia-Hidalgo, Javier ;
Acebal, Carmen ;
de la Mata, Isabel ;
Arroyo, Miguel .
BIORESOURCE TECHNOLOGY, 2012, 115 :177-182
[9]   Enzyme stabilization by nano/microsized hybrid materials [J].
Hwang, Ee Taek ;
Gu, Man Bock .
ENGINEERING IN LIFE SCIENCES, 2013, 13 (01) :49-61
[10]   Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solvent [J].
Hwang, Ee Taek ;
Lee, Byoungsoo ;
Zhang, Meiling ;
Jun, Seung-Hyun ;
Shim, Jongmin ;
Lee, Jinwoo ;
Kim, Jungbae ;
Gu, Man Bock .
GREEN CHEMISTRY, 2012, 14 (07) :1884-1887
12345