Preparation of Robust Biocatalyst Based on Cross-Linked Enzyme Aggregates Entrapped in Three-Dimensionally Ordered Macroporous Silica

被引:80
作者
Jiang, Yanjun [1 ]
Shi, Lianlian [1 ]
Huang, Yan [1 ]
Gao, Jing [1 ]
Zhang, Xu [1 ]
Zhou, Liya [1 ]
机构
[1] Hebei Univ Technol, Sch Chem Engn & Technol, Tianjin, Peoples R China
关键词
Three-dimensionally ordered macroporous materials; cross-linked enzyme aggregates; lipase; enzyme immobilization; biodiesel; SEPARABLE MESOPOROUS SILICA; CATALYTIC-PROPERTIES; BIODIESEL PRODUCTION; ORGANIC-SOLVENT; ALPHA-AMYLASE; IMMOBILIZATION; LIPASE; CLEAS; STABILIZATION; PERFORMANCE;
D O I
10.1021/am405104b
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
With the aim to provide a highly stable and active biocatalyst, cross-linked enzyme aggregates (CLEAs) of lipase Candida sp. 99-125 were prepared in three-dimensionally ordered macroporous silica materials (CLEAs-LP@ 3DOM-SiO2). Lipase Candida sp. 99-125 was first precipitated in the pores of 3DOM SiO2 (named EAs-LP@3DOM-SiO2), and further cross-linked by glutaraldehyde to form CLEAs-LP@3DOM-SiO2. Saturated ammonium sulfate was used as a precipitant and glutaraldehyde with a concentration of 0.25% (w/w) was employed as a cross-linker. Compared with EAs-LP@ 3DOM-SiO2 and native lipase, CLEAs-LP@3DOM-SiO2 exhibited excellent thermal and mechanical stability, and could maintain more than 85% of initial activity after 16 days of shaking in organic and aqueous phase. When CLEAs-LP@3DOM-SiO2 was applied in esterification and transesterification reactions, improved activity and reusability were achieved. This method can be used for the immobilization of other enzymes of interest.
引用
收藏
页码:2622 / 2628
页数:7
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