Functional analysis of Ralstonia solanacearum PrhG regulating the hrp regulon in host plants

被引:30
作者
Zhang, Yong [1 ]
Chen, Li [2 ]
Yoshimochi, Takeshi [3 ]
Kiba, Akinori [3 ]
Hikichi, Yasufumi [3 ]
Ohnishi, Kouhei [2 ]
机构
[1] Southwest Univ, Res Ctr Bioenergy & Bioremediat, Chongqing 400715, Peoples R China
[2] Kochi Univ, Res Inst Mol Genet, Nankoku, Kochi 7838502, Japan
[3] Kochi Univ, Lab Plant Pathol & Biotechnol, Nankoku, Kochi 7838502, Japan
来源
MICROBIOLOGY-SGM | 2013年 / 159卷
基金
日本学术振兴会;
关键词
III SECRETION SYSTEM; PSEUDOMONAS-SOLANACEARUM; GENES; EXPRESSION; VIRULENCE; PATHOGENICITY; COMPONENTS; INDUCTION; MOTILITY; INVASION;
D O I
10.1099/mic.0.067819-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genes in the hrp regulon encode component proteins of the type III secretion system and are essential for the pathogenicity of Ralstonia solanacearum. The hrp regulon is controlled by HrpB. We isolated several genes regulating hrpB expression from the Japanese strain OE1-1 using minitransposon mutagenesis. Among them, we mainly focused on two genes, hrpG and prhG, which are the positive regulators of hrpB. Although the global virulence regulator PhcA negatively regulated hrpG expression via prhIR, it positively regulated prhG expression. We further investigated the contrasting regulation of hrpG and prhG by PhcA and speculated that R. solanacearum may switch from HrpG to PrhG for hrpB activation in a cell density-dependent manner. Although the prhG mutant proliferated similarly to the wild-type in leaf intercellular spaces and in xylem vessels of the host plants, it was less virulent than the wild-type. The expression of the popA operon, which belongs to the hrp regulon, was significantly reduced in the prhG mutant by more than half in the leaf intercellular spaces and more than two-thirds in the xylem vessels when compared with the wild-type.
引用
收藏
页码:1695 / 1704
页数:10
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