DySCo: Quantitating Associations of Membrane Proteins Using Two-Color Single-Molecule Tracking

被引：58

作者：

Dunne, Paul D. ^{[4
]}

Fernandes, Ricardo A. ^{[1
,2
]}

McColl, James ^{[4
]}

Yoon, Ji Won ^{[3
]}

James, John R. ^{[1
,2
]}

Davis, Simon J. ^{[1
,2
]}

Klenerman, David ^{[4
]}

机构：

[1] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Clin Med, Oxford OX3 9DS, England

[2] Univ Oxford, John Radcliffe Hosp, MRC, Human Immunol Unit,Weatherall Inst Mol Med, Oxford OX3 9DS, England

[3] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

[4] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

来源：

BIOPHYSICAL JOURNAL | 2009年 / 97卷 / 04期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金; 英国医学研究理事会; 英国工程与自然科学研究理事会;

关键词：

T-CELL-RECEPTOR; LIVING CELLS; MICROSCOPY; COLOCALIZATION; DIFFUSION; NETWORKS; SURFACE;

D O I：

10.1016/j.bpj.2009.05.046

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We present a general method called dynamic single-molecule colocalization for quantitating the associations of single cell surface molecules labeled with distinct autofluorescent proteins. The chief advantages of the new quantitative approach are that, in addition to stable interactions, it is capable of measuring nonconstitutive associations, such as those induced by the cyloskeleton, and it is applicable to situations where the number of molecules is small.

引用

页码：L5 / L7

页数：3

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