Structure-Based Rational Design of a Phosphotriesterase

被引：31

作者：

Jackson, Colin J. ^{[1
]}

Weir, Kahli ^{[1
]}

Herlt, Anthony ^{[2
]}

Khurana, Jeevan ^{[1
]}

Sutherland, Tara D. ^{[1
]}

Horne, Irene ^{[1
]}

Easton, Christopher ^{[2
]}

Russell, Robyn J. ^{[1
]}

Scott, Colin ^{[1
]}

Oakeshott, John G. ^{[1
]}

机构：

[1] CSIRO Entomol, Canberra, ACT 2601, Australia

[2] Australian Natl Univ, Res Sch Chem, Acton, ACT 0200, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 15期

关键词：

ORGANOPHOSPHORUS HYDROLASE; BACTERIAL PHOSPHOTRIESTERASE; SUBSTRATE; SPECIFICITY; HYDROLYSIS;

D O I：

10.1128/AEM.00629-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (CVP) in the phosphotriesterase from Agrobacterium radiobacter identified two residues (F131 and W132) that prevent productive substrate binding and cause stereospecificity. A variant (W131H/F132A) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of Z-CVP and E-CVP hydrolysis, respectively, eliminating stereospecificity.

引用

页码：5153 / 5156

页数：4

共 17 条

[1] OpdA, a bacterial organophosphorus hydrolase, prevents lethality in rats after poisoning with highly toxic organophosphorus pesticides
Bird, Steven B.
Sutherland, Tara D.
Gresham, Chip
Oakeshott, John
Scott, Colin
Eddleston, Michael
[J]. TOXICOLOGY, 2008, 247 (2-3) : 88 - 92
[2] Structural determinants of the substrate and stereochemical specificity of phosphotriesterase
Chen-Goodspeed, M
Sogorb, MA
Wu, FY
Hong, SB
Raushel, FM
[J]. BIOCHEMISTRY, 2001, 40 (05) : 1325 - 1331
[3] Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents
Cho, CMH
Mulchandani, A
Chen, W
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (04) : 2026 - 2030
[4] Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos
Cho, CMH
Mulchandani, A
Chen, W
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (08) : 4681 - 4685
[5] DUMAS DP, 1989, J BIOL CHEM, V264, P19659
[6] Coot:: model-building tools for molecular graphics
Emsley, P
Cowtan, K
[J]. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 : 2126 - 2132
[7] A rigorous test for SPARC's chemical reactivity models: Estimation of more than 4300 ionization pK(a)s
Hilal, SH
Karickhoff, SW
Carreira, LA
[J]. QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIPS, 1995, 14 (04): : 348 - 355
[8] Metal-substrate interactions facilitate the catalytic activity of the bacterial phosphotriesterase
Hong, SB
Raushel, FM
[J]. BIOCHEMISTRY, 1996, 35 (33) : 10904 - 10912
[9] Identification of an opd (organophosphate degradation) gene in an Agrobacterium isolate
Horne, I
Sutherland, TD
Harcourt, RL
Russell, RJ
Oakeshott, JG
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (07) : 3371 - 3376
[10] The effects of substrate orientation on the mechanism of a phosphotriesterase
Jackson, CJ
Liu, JW
Coote, ML
Ollis, DL
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2005, 3 (24) : 4343 - 4350

← 1 2 →