Glucosinolate engineering identifies γ-glutamyl peptidase

被引：119

作者：

Geu-Flores, Fernando ^{[1
,2
]}

Nielsen, Morten Thrane ^{[1
,2
]}

Nafisi, Majse ^{[1
,2
]}

Moldrup, Morten Emil ^{[1
,2
]}

Olsen, Carl Erik ^{[2
,3
]}

Motawia, Mohammed Saddik ^{[1
,2
]}

Halkier, Barbara Ann ^{[1
,2
]}

机构：

[1] Univ Copenhagen, Fac Life Sci, Dept Plant Biol, Plant Biochem Lab, Frederiksberg, Denmark

[2] Univ Copenhagen, Fac Life Sci, Dept Plant Biol, VKR Res Ctr Proactive Plants, Frederiksberg, Denmark

[3] Univ Copenhagen, Fac Life Sci, Dept Nat Sci, Frederiksberg, Denmark

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 08期

关键词：

MYROSINASE SYSTEM; CANCER; SULFORAPHANE; GLUTATHIONE; PATHWAY;

D O I：

10.1038/nchembio.185

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Consumption of cruciferous vegetables is associated with reduced risk of developing cancer, a phenomenon attributed to glucosinolates, which are characteristic of these vegetables. We report production of the bioactive benzylglucosinolate in the noncruciferous plant Nicotiana benthamiana through metabolic engineering. The study includes identification of gamma-glutamyl peptidase 1 (GGP1), which substantially increased glucosinolate production by metabolizing an accumulating glutathione conjugate, an activity not previously described for glucosinolate biosynthesis or for proteins containing glutamine amidotransferase domains.

引用

页码：575 / 577

页数：3

共 19 条

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