Poly(dimethylsiloxane) microchip: microchannel with integrated open electrospray tip

被引：46
作者：
Svedberg, M
Veszelei, M
Axelsson, J
Vangbo, M
Nikolajeff, F
机构：
[1] Uppsala Univ, Angstrom Lab, Div Mat Sci, SE-75121 Uppsala, Sweden
[2] Amersham Biosci AB, Prot Separat, GE Healthcare, SE-75184 Uppsala, Sweden
[3] Uppsala Imanet, SE-75309 Uppsala, Sweden
来源：
LAB ON A CHIP | 2004年 / 4卷 / 04期
关键词：
D O I：
10.1039/b402490g
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A polymer microchip with an open tip for electrospray mass spectrometry is presented. The tip consists of a groove with parallel walls where a droplet can form at the end surface. A lid covers the whole chip except at the microchannel tip, which is left open. Poly( dimethylsiloxane) ( PDMS) microchips were cast using a nickel mould which in turn was replicated from a dry etched silicon wafer. Tips with microchannel widths of around 50 mm could easily be replicated. Since the tip had no cover, the assembly of microchip and cover was simplified. A total ion current variation of 5% during 300 s was achieved for a 1 muM myoglobin solution. The non-complex design of the cover makes it suitable for versatile tests of chip prototypes. The nickel mould was found to be useful for PDMS microstructure fabrication. Also, such a robust mould allows casting electrospray tips in more rigid thermoset materials.
引用
收藏
页码：322 / 327
页数：6
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