Cryptic polyadenylation sites within the coding sequence of three yeast genes expressed in tobacco

被引：12

作者：

Grec, S ^{[1
]}

Wang, YC ^{[1
]}

Le Guen, L ^{[1
]}

Negrouk, V ^{[1
]}

Boutry, M ^{[1
]}

机构：

[1] Univ Catholique Louvain, Unite Biochim Physiol, B-1348 Louvain, Belgium

来源：

GENE | 2000年 / 242卷 / 1-2期

关键词：

ABC transporter; DNA polymerase; plant; transgenic;

D O I：

10.1016/S0378-1119(99)00544-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Three yeast genes, MIP (mitochondrial DNA polymerase) and two genes, YCF1 (yeast cadmium factor 1) and PDR5 (pleiotropic drug resistance 5), conferring multidrug resistance, were provided with the cauliflower mosaic virus 35S transcription promoter and introduced into tobacco using an Agrobacterium tumefaciens T-DNA-derived vector. Transcripts of each gene much shorter than those expected were found in the transgenic plants. RT-PCR and S-1 nuclease mapping of the PDR5 and MIP transcripts demonstrated the presence of one (PDR5), or several close (MIP), cryptic polyadenylation site(s) within the coding sequence of these yeast genes. Possible sequences involved in polyadenylation are discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：87 / 95

页数：9

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