Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris

被引：1
|
作者：
Nguyen Hoang Loc [1 ]
Le My Tieu Ngoc [1 ]
Hoang Tan Quang [2 ]
Nguyen Duc Huy [2 ]
Nguyen Ngoc Luong [1 ]
机构：
[1] Hue Univ, Coll Sci, Hue 530000, Vietnam
[2] Hue Univ, Inst Biotechnol, Hue 530000, Vietnam
来源：
CHEMICAL PAPERS | 2016年 / 70卷 / 03期
关键词：
endo-beta-1,4-glucanase; expression; Glu1-TA; Trichoderma asperellum; HETEROLOGOUS EXPRESSION; SACCHAROMYCES-CEREVISIAE; MOLECULAR-CLONING; ASPERGILLUS-NIGER; BOMBYX-MORI; ENDOGLUCANASE; YEAST; EXO-BETA-1,3-GLUCANASE; PURIFICATION; VIRIDE;
D O I：
10.1515/chempap-2015-0210
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Two genes coding emlo-beta-1,TglucallaSCS were cloned In tin l'rich.oderma asperellun. PQ34 wh ch was isolated from Thua Thien Hue province, Vietnam. The expression of these genes in Pichia pastoris produced two enzymes with molecular masses of approximately 46 klla (about 42 klla enzyme and 4 kDa of signal peptide). The effects of induction tirne and temperature, inducer concentration, and culture medium on the endo-,3-1,4-glucanase activity were investigated. The results showed that the highest total activities 01 two endo-beta-1,4-gbicanases were approximately 4.7 x 10(-8) kat (from Glul-TA gene) and 7.3 x 10(-8) kat (from Glu2-TA gene) occurred after 4 days of induction using, 25 mL L-1 methanol at 30 degrees C when the yeast cells were cultured in a YPL medium (C) 2015 Institute of Chemistry, Slovak Academy of Sciences
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收藏
页码：284 / 293
页数：10
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