A Novel Approach to High-Quality Postmortem Tissue Procurement: The GTEx Project

被引：570

作者：

Carithers, Latarsha J. ^{[1
]}

Ardlie, Kristin ^{[2
]}

Barcus, Mary ^{[3
]}

Branton, Philip A. ^{[1
]}

Britton, Angela ^{[3
]}

Buia, Stephen A. ^{[3
]}

Compton, Carolyn C. ^{[1
]}

DeLuca, David S. ^{[2
]}

Peter-Demchok, Joanne ^{[1
]}

Gelfand, Ellen T. ^{[2
]}

Guan, Ping ^{[1
]}

Korzeniewski, Greg E. ^{[3
]}

Lockhart, Nicole C. ^{[1
]}

Rabiner, Chana A. ^{[1
]}

Rao, Abhi K. ^{[1
]}

Robinson, Karna L. ^{[3
]}

Roche, Nancy V. ^{[3
]}

Sawyer, Sherilyn J. ^{[1
]}

Segre, Ayellet V. ^{[2
]}

Shive, Charles E. ^{[3
]}

Smith, Anna M. ^{[3
]}

Sobin, Leslie H. ^{[3
]}

Undale, Anita H. ^{[3
]}

Valentino, Kimberly M. ^{[3
]}

Vaught, Jim ^{[1
]}

Young, Taylor R. ^{[2
]}

Moore, Helen M. ^{[1
]}

机构：

[1] NCI, Biorepositories & Biospecimen Res Branch, Canc Diag Program, NIH, Bethesda, MD 20892 USA

[2] Broad Inst MIT & Harvard, Cambridge, MA USA

[3] Leidos Biomed Res Inc, Clin Res Directorate, Biospecimen Res Grp, Frederick, MD USA

来源：

BIOPRESERVATION AND BIOBANKING | 2015年 / 13卷 / 05期

基金：

美国国家卫生研究院;

关键词：

HUMAN TRANSCRIPTOME;

D O I：

10.1089/bio.2015.0032

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Genotype-Tissue Expression (GTEx) project, sponsored by the NIH Common Fund, was established to study the correlation between human genetic variation and tissue-specific gene expression in non-diseased individuals. A significant challenge was the collection of high-quality biospecimens for extensive genomic analyses. Here we describe how a successful infrastructure for biospecimen procurement was developed and implemented by multiple research partners to support the prospective collection, annotation, and distribution of blood, tissues, and cell lines for the GTEx project. Other research projects can follow this model and form beneficial partnerships with rapid autopsy and organ procurement organizations to collect high quality biospecimens and associated clinical data for genomic studies. Biospecimens, clinical and genomic data, and Standard Operating Procedures guiding biospecimen collection for the GTEx project are available to the research community.

引用

页码：311 / 319

页数：9

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