Parallel manipulation of bifunctional DNA molecules on structured surfaces using kinesin-driven microtubules

被引：54

作者：

Dinu, Cerasela Zoica

Opitz, Joerg

Pompe, Wolfgang

Howard, Jonathon

Mertig, Michael ^{[1
]}

Diez, Stefan

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

[2] Dresden Univ Technol, Max Bergmann Ctr Biomat, BioNanotechnol & Struct Format Grp, D-01062 Dresden, Germany

[3] Dresden Univ Technol, Inst Mat Sci, D-01069 Dresden, Germany

来源：

SMALL | 2006年 / 2卷 / 8-9期

关键词：

DNA; kinesin; microtubules; nanomanipulation;

D O I：

10.1002/smll.200600112

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed a technique to manipulate bifunctional DNA molecules: One end is thiolated to bind to a patterned gold surface and the other end is biotinylated to bind to a microtubule gliding over a kinesin-coated surface. We found that DNA molecules can be stretched and overstretched between the gold pads and the motile microtubules, and that they can form dynamic networks. This serves as a proof-of-principle that biological machineries can be used in vitro to accomplish the parallel formation of structured DNA templates that will have applications in biophysics and nanoelectronics.

引用

页码：1090 / 1098

页数：9