An electrochemiluminescent aptasensor for amplified detection of exosomes from breast tumor cells (MCF-7 cells) based on G-quadruplex/hemin DNAzymes

被引：68

作者：

Qiao, Bin ^{[1
]}

Guo, Qunqun ^{[1
]}

Jiang, Juqian ^{[1
]}

Qi, Yunlong ^{[1
]}

Zhang, Hui ^{[1
]}

He, Bangshun ^{[2
]}

Cai, Chenxin ^{[1
]}

Shen, Jian ^{[1
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Key Lab Biomed Mat,Jiangsu Key Lab New Po, Jiangsu Collaborat Innovat Ctr Biomed Ilinct Mat, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Gen Clin Res Ctr, Nanjing Hosp 1, Nanjing 210006, Jiangsu, Peoples R China

来源：

ANALYST | 2019年 / 144卷 / 11期

基金：

中国国家自然科学基金;

关键词：

HYBRIDIZATION CHAIN-REACTION; ELECTROCHEMICAL DETECTION; EXTRACELLULAR VESICLES; SENSITIVE DETECTION; LABEL-FREE; DNA; AMPLIFICATION; QUANTIFICATION; NANOPARTICLES; BIOSENSOR;

D O I：

10.1039/c9an00181f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Exosomes are non-invasive biomarkers for cancer diagnosis. Herein, we describe an electrochemiluminescent (ECL) aptasensor for the detection of exosomes from breast tumor cells. Mercaptopropionic acid (MPA)-modified Eu3+-doped CdS nanocrystals (MPA-CdS:Eu NCs) and H2O2 were used as ECL emitters and coreactant, respectively. The exosomes are recognized and captured by the CD63 aptamer, and then form a G-quadruplex/hemin DNAzyme, which efficiently catalyzes the decomposition of H2O2, resulting in the decreased ECL signal of MPA-CdS: Eu NCs. The exosomes from breast tumor cells (MCF-7 cells) can be detected in the range of 3.4 x 10(5) to 1.7 x 10(8) particles per mL. The limit of detection (LOD) was estimated to be 7.41 x 10(4) particles per mL at a signal-to-noise ratio of 3. The aptasensor has been successfully used to detect exosomes in the serum.

引用

页码：3668 / 3675

页数：8

共 67 条

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