A SUPERVISION AND CONTROL TOOL BASED ON ARTIFICIAL INTELLIGENCE FOR HIGH CELL DENSITY CULTIVATIONS

被引：17

作者：

Horta, A. C. L. ^{[1
,2
]}

Silva, A. J. ^{[2
]}

Sargo, C. R. ^{[2
]}

Velez, A. M. ^{[2
]}

Gonzaga, M. C. ^{[2
]}

Giordano, R. C. ^{[2
]}

Goncalves, V. M. ^{[3
]}

Zangirolami, T. C. ^{[2
]}

机构：

[1] Univ Fed Goias, Inst Quim, BR-74001970 Goiania, Go, Brazil

[2] Univ Fed Sao Carlos, Dept Engn Quim, BR-13560 Sao Carlos, SP, Brazil

[3] Ctr Biotechnol, Inst Butantan, Sao Paulo, Brazil

来源：

BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING | 2014年 / 31卷 / 02期

基金：

巴西圣保罗研究基金会;

关键词：

High cell density cultivations; Monitoring and control system; Recombinant E. coli; Fed-batch; Artificial intelligence; RECOMBINANT ESCHERICHIA-COLI; PROTEIN-PRODUCTION; BIOLOGICAL CELLS; SURFACE PROTEIN; CULTURES; GROWTH; FERMENTATIONS; IMPROVE;

D O I：

10.1590/0104-6632.20140312s00002304

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

High cell density cultivations of recombinant E. coli have been increasingly used for the production of heterologous proteins. However, it is a challenge to maintain these cultivations within the desired conditions, given that some variables such as dissolved oxygen concentration (DOC) and feed flow rate are difficult to control. This paper describes the software SUPERSYS_HCDC, a tool developed to supervise fed-batch cultures of rE. coli with biomass concentrations up to 150 g(DCW)/L and cell productivities up to 9 g(DCW).L-1.h(-1). The tool includes automatic control of the DOC by integrated action of the stirrer speed as well as of the air and oxygen flow rates; automatic start-up of the feed flow of fresh medium (system based on a neural network committee); and automatic slowdown of feeding when oxygen consumption exceeds the maximum capacity of the oxygen supply.

引用

页码：457 / 468

页数：12

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