Degradation of tetracycline and oxytetracycline by crude lignin peroxidase prepared from Phanerochaete chrysosporium - A white rot fungus

被引：137

作者：

Wen, Xianghua ^{[1
]}

Jia, Yannan ^{[1
]}

Li, Jiaxi ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China

来源：

CHEMOSPHERE | 2009年 / 75卷 / 08期

关键词：

Lignin peroxidase; In vitro degradation; Tetracycline; Oxytetracycline; SOLID-PHASE EXTRACTION; PERSONAL CARE PRODUCTS; FLUOROQUINOLONE ANTIBACTERIAL AGENTS; SEWAGE-TREATMENT PLANT; WASTE-WATER; MANGANESE PEROXIDASE; LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; AQUATIC ENVIRONMENT; ESTROGENIC ACTIVITY;

D O I：

10.1016/j.chemosphere.2009.01.052

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Pharmaceuticals are becoming an emerging environmental issue that attracts increasing attention in recent years. This study chose tetracycline (TC) and oxytetracycline (OTC) as examples of pharmaceuticals. Crude lignin peroxidase (LiP) produced by Phanerochaete chrysosporium were used to degrade TC and OTC in vitro. The results illustrated that UP has a strong degrading ability towards TC and OTC. At 50 mg L-1 of TC and OTC and 40 U L-1 of the enzyme activity, the degradation of TC and OTC reached to about 95% in 5 min. The degradation of TC and OTC by UP was dependent on pH and temperature and was largely enhanced by increasing the concentrations of veratryl alcohol (VA) and initial hydrogen peroxide (H2O2). The optimized degradation conditions were determined as pH 4.2, 37 degrees C, 2 mM VA, 0.4 mM H2O2. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1003 / 1007

页数：5

共 44 条

[1] Comparison of the occurrence of antibiotics in four full-scale wastewater treatment plants with varying designs and operations [J].