Confinement Spectroscopy: Probing Single DNA Molecules with Tapered Nanochannels

被引：116

作者：

Persson, Fredrik ^{[1
]}

Utko, Pawel ^{[1
]}

Reisner, Walter ^{[1
]}

Larsen, Niels B. ^{[1
]}

Kristensen, Anders ^{[1
]}

机构：

[1] Tech Univ Denmark, DTU Nanotech, Dept Micro & Nanotechnol, DK-2800 Kongens Lyngby, Denmark

来源：

NANO LETTERS | 2009年 / 9卷 / 04期

关键词：

FORCE; LENGTH;

D O I：

10.1021/nl803030e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate a confinement spectroscopy technique capable of probing small conformational changes of unanchored single DNA molecules in a manner analogous to force spectroscopy, in the regime corresponding to femtonewton forces. In contrast to force spectroscopy, various structural forms of DNA can easily be probed, as indicated by experiments on linear and circular DNA. The extension of circular DNA is found to scale according to the de Gennes exponent, unlike for linear DNA.

引用

页码：1382 / 1385

页数：4

共 27 条

[1] OPTICAL TRAPPING AND MANIPULATION OF SINGLE CELLS USING INFRARED-LASER BEAMS
ASHKIN, A
DZIEDZIC, JM
YAMANE, T
[J]. NATURE, 1987, 330 (6150) : 769 - 771
[2] Do femtonewton forces affect genetic function? A review
Blumberg, Seth
Pennington, Matthew W.
Meiners, Jens-Christian
[J]. JOURNAL OF BIOLOGICAL PHYSICS, 2006, 32 (02) : 73 - 95
[3] Ten years of tension: single-molecule DNA mechanics
Bustamante, C
Bryant, Z
Smith, SB
[J]. NATURE, 2003, 421 (6921) : 423 - 427
[4] De Gennes P.-G., 1979, SCALING CONCEPTS POL
[5] Doi M., 1986, THEORY POLYM DYNAMIC
[6] SENSING SPECIFIC MOLECULAR-INTERACTIONS WITH THE ATOMIC-FORCE MICROSCOPE
FLORIN, EL
RIEF, M
LEHMANN, H
LUDWIG, M
DORNMAIR, C
MOY, VT
GAUB, HE
[J]. BIOSENSORS & BIOELECTRONICS, 1995, 10 (9-10) : 895 - 901
[7] GRAYSON P, 2007, THESIS CALTECH PASA
[8] DNA and its associated processes as targets for cancer therapy
Hurley, LH
[J]. NATURE REVIEWS CANCER, 2002, 2 (03) : 188 - 200
[9] KRISHNAN M, ARXIV08052100V1
[10] STRUCTURAL CONSIDERATIONS IN INTERACTION OF DNA AND ACRIDINES
LERMAN, LS
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1961, 3 (01) : 18 - &

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