Up-regulation of TRAF2 inhibits chondrocytes apoptosis in lumbar facet joint osteoarthritis

被引：13

作者：

Jiang, Jiawei ^{[1
]}

Zhang, Jinlong ^{[1
]}

Wu, Chunshuai ^{[1
]}

Guo, Xiaofeng ^{[1
]}

Chen, Chu ^{[1
]}

Bao, Guofeng ^{[1
]}

Sun, Yuyu ^{[1
]}

Chen, Jiajia ^{[1
]}

Xue, Pengfei ^{[1
]}

Xu, Guanhua ^{[1
]}

Cui, Zhiming ^{[1
]}

机构：

[1] Nantong Univ, Affiliated Hosp 2, Dept Spine Surg, Nantong 226001, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 03期

关键词：

TRAF2; Lumbar facet joint; Osteoarthritis; Apoptosis; Tumor necrosis factor-alpha; TUMOR-NECROSIS-FACTOR; NF-KAPPA-B; SIGNAL-TRANSDUCTION; GENE-EXPRESSION; MAP KINASES; CELL-DEATH; TNF-ALPHA; RECEPTOR; INDUCTION; TRADD-TRAF2;

D O I：

10.1016/j.bbrc.2018.07.096

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tumor necrosis factor receptor-associated factor 2 (TRAF2) has been demonstrated that it plays a significant role in cell death receptor signal transduction. The purpose of this study was to investigate the expression of TRAF2 and its possible role in FJOA. We observed an up-regulation of TRAF2 in FJOA by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) compared to normal tissues. In vitro, we used TNF-alpha to stimulate Human SW1353 chondrosarcoma cells to establish the chondrocytes injury model. Western blot analysis revealed significant expression of TRAF2 and cleaved caspase-3/8 in SW1353 cells. Co-localization of TRAF2/cleaved caspase-3/8 was detected in the cells injury model by double-labeling immunofluorescent staining. We demonstrated a possible anti-apoptotic effect of TRAF2 in chondrocyte apoptosis in FJOA by knockdown of its expression with siRNA. Moreover, TRAF2 knockdown was demonstrated to enhance TNF-alpha-induced apoptosis by flow cytometry assay. In conclusion, our results show that the up-regulation of TRAF2 may play an important role in the inhibition of chondrocyte apoptosis of FJOA. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：1659 / 1665

页数：7

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