Solid-state nanopore analysis of alcohol-soluble molecules

被引：0
作者：
Roy, Dhrubajyoti Basu [1 ,3 ]
Hall, Adam R. [1 ,2 ]
机构：
[1] Wake Forest Univ, Sch Med, Virginia Tech Wake Forest Sch Biomed Engin & Sci, Winston Salem, NC 27101 USA
[2] Wake Forest Univ, Sch Med, Comprehens Canc Ctr, Winston Salem, NC 27101 USA
[3] Beckman Res Inst, Dept Mol Med, Duarte, CA 91010 USA
来源：
ANALYST | 2017年 / 142卷 / 10期
关键词：
DNA TRANSLOCATION; ALPHA-ZEIN; PROTEINS; SINGLE; TRANSPORT;
D O I：
10.1039/c7an00022g
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report on a modified solid-state nanopore measurement scheme to probe alcohol-soluble proteins. Taking advantage of the intrinsic alcohol solubility of LiCl as an electrolyte, we show that the devices can be operated in azeotropic mixtures of ethanol and water. We first characterize nanopore conductivity across a range of ethanol content as a function of both nanopore diameter and salt concentration, showing ionic response that can be understood through established models. Then, as a demonstration of resistive-pulse sensing, we measure and interpret electrical translocations of zeins, a class of alcohol-soluble maize protein.
引用
收藏
页码：1676 / 1681
页数：6
相关论文
共 50 条
  • [31] Dynamics of a Molecular Plug Docked onto a Solid-State Nanopore
    Shi, Xin
    Li, Qiao
    Gao, Rui
    Si, Wei
    Liu, Shao-Chuang
    Aksimentiev, Aleksei
    Long, Yi-Tao
    JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2018, 9 (16): : 4686 - 4694
  • [32] Direct observation of DNA knots using a solid-state nanopore
    Plesa, Calin
    Verschueren, Daniel
    Pud, Sergii
    van der Torre, Jaco
    Ruitenberg, Justus W.
    Witteveen, Menno J.
    Jonsson, Magnus P.
    Grosberg, Alexander Y.
    Rabin, Yitzhak
    Dekker, Cees
    NATURE NANOTECHNOLOGY, 2016, 11 (12) : 1093 - 1097
  • [33] The temporal resolution and single-molecule manipulation of a solid-state nanopore by pressure and voltage
    Zhang, Hengbin
    Chen, Qiang
    Wu, Yaojun
    Wang, Yangyang
    Bei, Xiaomin
    Xiao, Lin
    NANOTECHNOLOGY, 2018, 29 (49)
  • [34] A Low-Noise Solid-State Nanopore Platform Based on a Highly Insulating Substrate
    Lee, Min-Hyun
    Kumar, Ashvani
    Park, Kyeong-Beom
    Cho, Seong-Yong
    Kim, Hyun-Mi
    Lim, Min-Cheol
    Kim, Young-Rok
    Kim, Ki-Bum
    SCIENTIFIC REPORTS, 2014, 4
  • [35] Fabrication of solid-state nanopores
    Lin, Kabin
    Chen, Chen
    Wang, Congsi
    Lian, Peiyuan
    Wang, Yan
    Xue, Song
    Sha, Jingjie
    Chen, Yunfei
    NANOTECHNOLOGY, 2022, 33 (27)
  • [36] Label-free analysis of physiological hyaluronan size distribution with a solid-state nanopore sensor
    Rivas, Felipe
    Zahid, Osama K.
    Reesink, Heidi L.
    Peal, Bridgette T.
    Nixon, Alan J.
    DeAngelis, Paul L.
    Skardal, Aleksander
    Rahbar, Elaheh
    Hall, Adam R.
    NATURE COMMUNICATIONS, 2018, 9
  • [37] Synthetic heparan sulfate standards and machine learning facilitate the development of solid-state nanopore analysis
    Xia, Ke
    Hagan, James T.
    Fu, Li
    Sheetz, Brian S.
    Bhattacharya, Somdatta
    Zhang, Fuming
    Dwyer, Jason R.
    Linhardt, Robert J.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2021, 118 (11)
  • [38] Effect of charge patterns along a solid-state nanopore on polyelectrolyte translocation
    Katkar, H. H.
    Muthukumar, M.
    JOURNAL OF CHEMICAL PHYSICS, 2014, 140 (13)
  • [39] Making of a single solid-state nanopore on the wall of fused silica capillary
    Fang, Fang
    He, Yan-Qin
    Tian, Li
    Li, Yun-Yun
    Wu, Zhi-Yong
    ROYAL SOCIETY OPEN SCIENCE, 2018, 5 (06):
  • [40] Solid-state nanopore localization by controlled breakdown of selectively thinned membranes
    Carlsen, Autumn T.
    Briggs, Kyle
    Hall, Adam R.
    Tabard-Cossa, Vincent
    NANOTECHNOLOGY, 2017, 28 (08)
12345