U-shaped, double-tapered, fiber-optic sensor for effective biofilm growth monitoring

被引:54
作者
Zhong, Nianbing [1 ]
Zhao, Mingfu [1 ]
Li, Yishan [2 ]
机构
[1] Chongqing Univ Technol, Chongqing Key Lab Modern Photoelect Detect Techno, Chongqing 400054, Peoples R China
[2] Chongqing Univ, Minist Educ, Key Lab Low Grade Energy Utilizat Technol & Syst, Chongqing 400030, Peoples R China
基金
中国国家自然科学基金;
关键词
HYDROGEN-PRODUCTION; REFRACTIVE-INDEX; PHOTOBIOREACTOR; FABRICATION; ACCUMULATION; SPECTROSCOPY; TEMPERATURE; PERFORMANCE; MICROSCOPY; MECHANISM;
D O I
10.1364/BOE.7.000352
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To monitor biofilm growth on polydimethylsiloxane in a photobioreactor effectively, the biofilm cells and liquids were separated and measured using a sensor with two U-shaped, double-tapered, fiber-optic probes (Sen. and Ref. probes). The probes' Au-coated hemispherical tips enabled double-pass evanescent field absorption. The Sen. probe sensed the cells and liquids inside the biofilm. The polyimide-silica hybrid-film-coated Ref. probe separated the liquids from the biofilm cells and analyzed the liquid concentration. The biofilm structure and active biomass were also examined to confirm the effectiveness of the measurement using a simulation model. The sensor was found to effectively respond to the biofilm growth in the adsorption through exponential phases at thicknesses of 0-536 mu m. (C) 2016 Optical Society of America
引用
收藏
页码:352 / 368
页数:17
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