Protection of pigs against classical swine fever with DNA-delivered gp55

被引：46

作者：

Andrew, ME ^{[1
]}

Morrissy, CJ ^{[1
]}

Lenghaus, C ^{[1
]}

Oke, PG ^{[1
]}

Sproat, KW ^{[1
]}

Hodgson, ALM ^{[1
]}

Johnson, MA ^{[1
]}

Coupar, BEH ^{[1
]}

机构：

[1] CSIRO, Anim Hlth, Geelong, Vic 3220, Australia

来源：

VACCINE | 2000年 / 18卷 / 18期

关键词：

DNA vaccination; classical swine fever; protection;

D O I：

10.1016/S0264-410X(99)00445-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Classical swine fever virus causes significant mortality and morbidity in commercial piggeries in many countries in Europe and Asia. The protective antigen, gp55, is highly conformation-dependent and thus killed virus or bacterially produced proteins are not protective. This report demonstrates that DNA vaccination with the gene encoding gp55 can provide protective immunity with inoculation of two doses of 25 mu g DNA or a single shot of 200 mu g. Furthermore, the DNA can be delivered intramuscularly or by a simple spring-loaded needleless inoculator. In addition it is shown that inoculation of the DNA at a single site conveys the same level of immunity as division of the dose between two sites. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1932 / 1938

页数：7

共 21 条

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