Direct identification of mycobacteria from smear-positive samples using real-time polymerase chain reaction

被引：3

作者：

Marekovic, I. ^{[1
,2
]}

Bosnjak, Z. ^{[2
]}

Plecko, V. ^{[1
,2
]}

机构：

[1] Univ Zagreb, Sch Med, Zagreb 41001, Croatia

[2] Univ Hosp Ctr Zagreb, Dept Clin & Mol Microbiol, Zagreb 10000, Croatia

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 08期

关键词：

acid-fast bacilli grade; bronchoalveolar lavage; tuberculosis; SPUTUM SAMPLES; PCR; TUBERCULOSIS;

D O I：

10.5588/ijtld.13.0042

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The aim of the present study was to investigate whether real-time polymerase chain reaction (PCR) has a low identification rate for samples with low acid-fast bacilli (AFB) grades, including those obtained using bronchoscopy. When 50 smear-positive samples were compared by AFB score and PCR result, PCR was 100% successful in identifying AFB 2+ and AFB 3+ samples. However, only 14 of 26(52%) AFB 1+ samples were identified. In paucibacillary smear-positive samples, PCR is not reliable enough to exclude tuberculosis, but in smear-positive patients with high AFB grades PCR can immediately change the clinical management of the disease.

引用

页码：978 / 980

页数：3

共 50 条

[41] Clarifying Indeterminate Results on the Rabies Direct Fluorescent Antibody Test Using Real-Time Reverse Transcriptase Polymerase Chain Reaction
Appler, Kim
Brunt, Scott
Jarvis, Jodie A.
Davis, April D.
PUBLIC HEALTH REPORTS, 2019, 134 (01) : 57 - 62
[42] Assessment of Prevalence of Non-tuberculous Mycobacteria in Archival Acid-fast Bacilli Positive Smear Slides by TaqMan Real-time PCR Assay
Farivar, Taghi Naserpour
Johari, Pouran
Moien, Abbas Ali
Shahri, Mohammad Hashemi
Naderi, Mohammad
Oskouie, Hamid
NORTH AMERICAN JOURNAL OF MEDICAL SCIENCES, 2012, 4 (05) : 231 - 234
[43] Effectiveness of real-time polymerase chain reaction assay for the detection of Mycobacterium tuberculosis in pathological samples: a systematic review and meta-analysis
Emmanuel O. Babafemi
Benny P. Cherian
Lee Banting
Graham A. Mills
Kandala Ngianga
Systematic Reviews, 6
[44] Comparison of examination of milk samples using the real-time polymerase chain reaction assay Mastit 4A and enriched bacteriological culturing
Soltau, Jennifer
Einax, Esra
Zoche-Golob, Veit
Klengel, Karin
Katholm, Jurgen
Donat, Karsten
Wehrend, Axel
BERLINER UND MUNCHENER TIERARZTLICHE WOCHENSCHRIFT, 2018, 131 (3-4): : 108 - 115
[45] Quantification of Hazelnut Content in Fillings of Cakes and Wafers Using Real-Time Polymerase Chain Reaction
Janska, V.
Piknova, L.
Kuchta, T.
FOOD ANALYTICAL METHODS, 2013, 6 (05) : 1492 - 1495
[46] Quantification of Hazelnut Content in Fillings of Cakes and Wafers Using Real-Time Polymerase Chain Reaction
V. Janská
L. Piknová
T. Kuchta
Food Analytical Methods, 2013, 6 : 1492 - 1495
[47] A real-time polymerase chain reaction assay for detection of Pneumocystis from bronchoalveolar lavage fluid
Arcenas, RC
Uhl, JR
Buckwalter, SP
Limper, AH
Crino, D
Roberts, GD
Wengenack, NL
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 54 (03) : 169 - 175
[48] Fast detection and quantification of four dairy propionic acid bacteria in milk samples using real-time quantitative polymerase chain reaction
Turgay, Meral
Schaeren, Walter
Wechsler, Daniel
Butikofer, Ueli
Graber, Hans Ulrich
INTERNATIONAL DAIRY JOURNAL, 2016, 61 : 37 - 43
[49] SARS-CoV-2 direct real-time polymerase chain reaction testing in laboratories with shortage challenges
Alaifan, Taghreed
Altamimi, Asmaa
Obeid, Dalia
Alshehri, Turki
Almatrrouk, Shaihana
Albarrag, Ahmed
FUTURE VIROLOGY, 2021, 16 (02) : 133 - 140
[50] Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Gallo, Juliana Failde
Watanabe Pinhata, Juliana Maira
Chimara, Erica
Goncalves, Maria Gisele
Fukasawa, Lucila Okuyama
de Oliveira, Rosangela Siqueira
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2016, 111 (09): : 545 - 550

← 1 2 3 4 5 →